摘要
目的:研究麻黄附子细辛汤与抗生素联合应用对抗生素的增效作用,为研究中药对抗生素增效的作用机制和筛选活性成分奠定实验基础。方法:将麻黄附子细辛汤复方、中药复方加头孢唑林、头孢唑林分别加入培养基中,同时制作未加药物的空白培养基作为空白组和阴性组,以及单纯加入替加环素的培养基作为阳性组,分别接种肺炎链球菌后37℃培养过夜,空白组不接种细菌,每12 h取出计数,计算活菌数;同时使用连续稀释法检测其各自最小抑菌浓度(MIC)及最低杀菌浓度(MBC);并且用纸片法检测抑菌圈。结果:1)中药复方组12 h时发现细菌逐渐生长,直至培养120 h后细菌浓度才逐渐下降,直至培养180 h时细菌浓度保持不变,头孢唑林组培养12 h时细菌数逐渐减少,到60 h时细菌减少的趋势停止,中药复方联合头孢唑林组培养12 h时细菌数逐渐减少,并在培养144 h时各组细菌均无生长迹象,替加环素药物干预后细菌数急剧下降,但是直到观察结束仍有部分细菌存活,阴性组细菌随着培养时间的逐渐延长呈现缓慢生长趋势,空白组无细菌生长。2)麻黄附子细辛汤对肺炎链球菌(MIC=1.25 mg/m L,MBC=2.5 mg/m L);头孢唑林对肺炎链球菌(MIC=0.625 mg/m L,MBC=1.25 mg/m L);中药复方联合头孢唑林对肺炎链球菌(MIC=0.312 mg/m L,MBC=1.25 mg/m,);替加环素对肺炎链球菌(MIC=0.156 mg/m L,MBC=1.25 mg/m L)。3)中药复方联合头孢唑林抑菌环直径和阳性对照组相近,其次是头孢唑林组,中药单独使用的抑菌环直径小于头孢唑林组,麻黄附子细辛汤与头孢唑林联合用药对肺炎链球菌的抗菌效应均增强,麻黄附子细辛汤与头孢唑林联合用药的抗茵作用表现为协同作用(FIC=0.59)。结论:麻黄附子细辛汤联合抗生素具有更明显的抗菌效应,可能与它激活耐药细菌生长有关。
Objective： To study the synergistic effect of Mahuang Fuzi Xixin Decoction combined with antibiotics on single-used antibiotics,and thus to lay the experimental foundation to screen active ingredients. Methods： The ingredients of Mahuang Fuzi Xixin Decoction,the compound Chinese medicine combined cefazolin,and cefazolin were added into the tissue mediums respectively as experimental groups,a blank tissue medium was taken as a blank control group as well as the negative group,and a tissue medium added sorely with Tigecycline was taken as the positive group. Except for the blank group,inoculation of s. pneumoniae was given to each tissue medium and was cultured at 37 ℃ overnight. Every 12 hours,the mediums were taken out for recording viable counts,and minimal inhibitory concentration（ MIC） and minimum bactericidal concentration（ MBC） of the bacteria were detected by continuous dilution method,and inhibition zones were detected by paper slip method. Results： 1） It was found that bacteria grew gradually in the Traditional Chinese Medicine compound group at 12 h,and after cultured for 120 h,the bacteria concentration gradually decreased. Until cultured for 180 h,the bacteria concentration started to remain the same. In cefazolin group,bacteria count reduced gradually in the first 12 h,the downtrend of bacteria ended at 60 h. In Traditional Chinese Medicine compound combined cefazolin group,the bacteria count reduced gradually at the first 12 h,and after 144 h of culture,all groups of bacteria showed no sign of growth at the same time,with Tigecycline intervation bacterial count fell sharply. However,some bacteria still survive to the end of the observation. The negative groups bacteria grew slowly along culture and the bacteria of blank control group showed no sign of bacteria. 2） The Mhuang Fuzi Xixin Decoction vs. s. pneumoniae（ MIC = 1. 25 mg / ml,MBC =2. 5mg / ml）; Cefazolin vs. s. pneumoniae（ MIC = 0. 625 mg / ml,MBC = 1. 25 mg / ml）; Traditional Chinese medicine compound combined cefazolin vs. s. pneumoniae（ MIC = 0. 312 mg / ml,MBC = 1. 25 mg / ml）; Tigecycline vs. s. pneumoniae（ MIC =0. 156 mg / ml,MBC = 1. 25 mg / ml）. 3） Traditional Chinese Medicine compound combined cefazolin group had close Bacteriostasis Circle diameter with the positive control group,and the second was cefazolin group. Traditional Chinese Medicine used alone had shorter Bacteriostasis Circle diameter than cefazolin group. The Mahuang Fuzi Xixin Decoction compound with cefazolin combination had enhanced antibacterial effect on streptococcus pneumoniae,and it has synergy effect（ FIC = 0. 59）. Conclusion： The Mahuang Fuzi Xixin combined antibiotics have more obvious antibacterial effect,the reason for this might be it activates the growth of resistant bacteria.
出处
《世界中医药》
CAS
2015年第6期891-894,899,共5页
World Chinese Medicine
基金
四川省中医管理局项目(编号:2012-E042)
国家自然科学基金面上项目(编号:81473669)
关键词
麻黄附子细辛汤
抗生素
最小抑菌浓度
最低杀菌浓度
细菌生长
Mahuang Fuzi Xixin Decoction
S.pneumoniae antibiotics
Minimal inhibitory concentration
Minimum bactericidal concentration
Bacterial growth
