摘要
本研究旨在建立一种变波长高效液相色谱法同时测定糕点中5种食品添加剂的快速检测方法。最终确定分离条件为博纳艾杰尔Venusil XBP Perservatives色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇和20 mmol·L^(-1)磷酸二氢铵(pH值调至5.5)进行梯度洗脱,DAD紫外检测器(190~400 nm)进行变波长监测,外标法进行定量分析。结果表明,5种食品添加剂的回收率为95.44%~96.96%,精密度为0.10%~0.20%。
This study aims to establish a rapid detection method for five food additives in pastries using variable wavelength high-performance liquid chromatography.The final separation condition was determined to be the Bonna-Agela’s Venusil XBP Preservatives column(4.6 mm×250 mm,5μm),The mobile phase consists of methanol and 20 mmol·L^(-1) ammonium dihydrogen phosphate(pH value adjusted to 5.5)for gradient elution.The DAD ultraviolet detector(190~400 nm)is used for wavelength monitoring,and the external standard method is used for quantitative analysis.The result showed that recovery rate range of five food additives is 95.44%~96.96%,the precision range is 0.10%~0.20%.
作者
李俊运
李丽
LI Junyun;LI Li(College of Bioengineering,Sichuan University of Science&Engineering,Yibin 644005,China;Yibin Food and Drug Inspection and Testing Center,Yibin 644600,China)
出处
《食品安全导刊》
2024年第3期65-67,共3页
China Food Safety Magazine
关键词
食品添加剂
糕点
变波长
高效液相色谱法
food additives
pastries
variable wavelength
high-performance liquid chromatography
