摘要
株型是影响作物产量和成本投入的重要因素,挖掘株型相关的QTLs将为黄瓜株型改良提供重要参考。以野生黄瓜PI183967为母本,以新泰密刺纯系为父本构建了含129个株系的重组自交系群体,对黄瓜株型的相关性状进行表型调查;同时基于亲本和重组自交系群体的重测序数据构建遗传连锁图谱,进而对黄瓜株型相关性状进行QTL定位分析。结果表明:基于重测序数据构建了包含598个bin标记的遗传连锁图谱,该图谱的遗传总长为1353 cM,标记间物理距离为352.8 kb;对株高、节间长度、节位数、侧枝数量和茎粗进行QTL分析,共定位到7个株型相关的QTLs,其中株高、节间长度各2个,节位数、侧枝数量、茎粗各1个;通过参考注释信息和查阅文献,初步筛选到9个可能与黄瓜株型相关的候选基因。
Plant architecture is a significant factor affecting yield and cost input in agricultural production.Mapping QTLs related to plant architecture in cucumber will provide essential reference for genetic improvement of cucumber architecture.In this work,129 recombinant inbred lines which originated from a narrow-cross between cucumber cultivar‘Xintaimici’and wild cucumber‘PI183967’was constructed,and phenotypic investigation related to cucumber architecture was also conducted.At the same time,genetic linkage map was constructed based on resequencing data of parents and recombinant inbred lines,and then QTL mapping analysis related cucumber architecture was performed.The results indicated that the genetic linkage map containing 598 bin markers was constructed,the genetic linkage map’s overall distance is 1353 cM,and the average physical distance between two bin markers is 352.8 kb.QTL mapping analysis for plant height,internode length,internode number,branch number and Stem diameter was conducted.A total of 7 QTLs were detected,including 2 QTLs for plant height,2 QTLs for internode length,1 QTL for internode number,1 QTL for branch number and 1 QTL for stem diameter.Nine causative genes preliminarily were filtered and predicted by the annotated data and literature.
作者
郭江怡
辛同旭
张梦卓
王帅
王深浩
杨学勇
GUO Jiangyi;XIN Tongxu;ZHANG Mengzhuo;WANG Shuai;WANG Shenhao;YANG Xueyong(College of Horticulture,Qingdao Agricultural University,Qingdao 266109,Shandong,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,State Key Laboratory of Vegetable Biobreeding,Beijing 100081,China)
出处
《中国蔬菜》
北大核心
2023年第8期38-45,共8页
China Vegetables
基金
中国农业科学院科技创新工程项目(CAAS-ASTIP)。
关键词
黄瓜
株型
重组自交系
QTL
cucumber
plant architecture
recombinant inbred lines
QTL
