摘要
目的:获得天冬Asparagus cochinchinensis(Lour.)Merr.全长转录组数据库,为探索其活性成分生物合成提供参考。方法:利用RNA-Seq技术对天冬的根、茎、叶进行转录组测序并分析。结果:从天冬转录组中共获得169319条isoforms,最终有147762条isoforms在公共数据库中注释成功。非冗余蛋白(NR)数据库结果显示,天冬与石刁柏Asparagus officinalis L.同源性最高;真核生物相邻类的聚簇(KOG)功能注释信息334380条,可分为25个基因功能大类;京都基因与基因组百科全书(KEGG)通路富集分析显示,代谢相关的通路分布最多,为74095条。在注释到的54个转录因子家族中,MYB家族数目最多,共309条。在所有isoform中共检测到48042个简单重复序列位点,从单核苷酸到六核苷酸重复单元均有分布。结论:获得了较为可靠的天冬全长转录组数据,可为深入研究天冬生物学特性、相关代谢途径、信号通路及其分子机制提供数据支持。
Objective:To obtain a full-length transcriptome database of Asparagus cochinchinensis(Lour.)Merr.in order to explore the biosynthesis of its active components.Methods:RNA-Seq technology was used to perform transcriptome sequencing of mixed samples from the root,stem,leaf of A.cochinchinensis.Results:A total of 169319 isoforms were obtained from the transcriptome of A.cochinchinensis,and 147762 isoforms were successfully annotated in the public database.NR results showed that A.cochinchinensis had the highest homology with A.officinalis L.KOG functional annotation yielded 334380 entries,which could be classified into 25 gene functional categories.KEGG enrichment analysis indicated that the metabolic pathways were the most abundant,accounting for 74095 entries.Among the 54 annotated transcription factor families,the MYB family had the highest number with a total of 309 entries.A total of 48042 simple repetitive sequence loci were detected in all isoforms,ranging from mononucleotide to hexanucleotide repeat units.Conclusion:A reliable complete transcriptome dataset of A.cochinchinensis has been obtained,providing data support for in-depth studies on the biological characteristics,related metabolic pathways,signaling pathways,and molecular mechanisms of A.cochinchinensis.
作者
陈芷宁
韦范
韦坤华
梁莹
胡营
韦筱媚
林杨
汤丹峰
缪剑华
CHEN Zhi-ning;WEI Fan;WEI Kun-hua;LIANG Ying;HU Ying;WEI Xiao-mei;LIN Yang;TANG Dan-feng;MIAO Jian-hua(Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement,Guangxi Engineering Research Center of TCM Resource Intelligent Creation,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China;School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China)
出处
《中国现代中药》
CAS
2023年第7期1496-1504,共9页
Modern Chinese Medicine
基金
广西创新驱动发展专项(桂科AA18242040)
广西科技计划项目(桂科AD22080012)
对发展中国家科技援助项目(KY201904001)。
关键词
天冬
全长转录组
代谢途径
转录因子
Asparagus cochinchinensis(Lour.)Merr.
full-length transcriptome
metabolism pathway
transcription factor
