摘要
以野生型西瓜优良自交系302为母本,利用化学诱变剂甲基磺酸乙酯(ethyl methane sulfonate,EMS)诱变获得的西瓜短节间突变体si302为父本,构建6世代分离群体,进行植株表型性状鉴定和短节间性状遗传分析;并通过极端性状混池重测序(bulked segregant analysis sequencing,BSA-seq)技术对突变基因进行初步定位。植株表型分析结果表明,突变体si302株型紧凑,主蔓长度和节间长度显著短缩,花器和果实均发育正常,符合西瓜简约化栽培株型要求;细胞形态学观察表明,si302茎蔓节间细胞长度缩短。遗传分析结果表明,F2群体野生型和突变体植株分离比例符合3∶1(χ^(2)=0.16),突变体si302短节间性状由1对隐性基因控制;根据BSA-seq测序结果,将该基因初步定位在1号染色体36.0~36.7 Mb区间。
We obtained a short internode mutant si302 by ethyl methane sulfonate(EMS)mutagenesis of watermelon inbred line 302.In this study,we collected the agronomic traits of six-basic generations(P1,P2,F1,F2,BC1P1 and BC1P2)from the cross between 302 and si302.Genetic analysis and bulked segregant analysis sequencing(BSA-seq)were used to located the candidate genes for the short internode trait in si302.Results showed that plant height,stem length and stem internodes of si302 were decreased than 302.But si302 had compacted plant shape and normal fruit traits paraffin section showed that the si302 internode cell length is shorter than 302.Genetic analysis showed that the si302 mutant is inherited in a 3∶1 ratio(χ^(2)=0.16),the short internode mutant phenotype of si302 was controlled by one recessive gene.With the strategy of BSA-seq,the short internode mutant was primary mapped into 36.0-36.7 Mb region on chromosome 1.
作者
马彩娥
肖艳红
柳唐镜
宫国义
张洁
张海英
MA Cai’e;XIAO Yanhong;LIU Tangjing;GONG Guoyi;ZHANG Jie;ZHANG Haiying(College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China;Vegetable Research Institute,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Horticultural Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China)
出处
《中国蔬菜》
北大核心
2023年第7期33-38,共6页
China Vegetables
基金
北京市农林科学院创新能力建设项目(KJCX201907-2)
国家西甜瓜产业技术体系项目(CARS-25)
北京市特色作物创新团队项目(BAIC04-2023)
广西壮族自治区八桂学者项目(2016A11)。
关键词
西瓜
短节间突变体
表型分析
BSA-seq
基因定位
watermelon
short-internode mutant
phenotypic analysis
bulked segregant analysis sequencing(BSA-seq)
gene mapping
