摘要
籽粒重量是大豆产量构成的关键因素之一,克隆主要数量性状基因座(QTL)中控制种子重量的关键基因对提高大豆产量具有重要意义。本研究以齐黄34×东生16构建的325个重组自交系(RIL)为材料,利用SLAF-seq构建了高密度遗传连锁图谱,总图距为2945.26 cM,平均图距为0.47 cM,结合3个环境百粒重表型,检测到11个与百粒重相关的QTL。其中,环境稳定的QTL为qSW20-1,可解释9.73%~18.10%的表型变异。该QTL的大粒等位基因可显著增加单株粒数和单株粒重,但对蛋白含量和脂肪含量2个品质性状无不利影响,区间大小为435.42kb,包含36个基因,通过基因注释和表达模式分析,预测5个候选基因。本研究结果为大豆增产基因挖掘和分子设计育种奠定了坚实的基础。
Seed weight is one of the key factors of soybean yield.Cloning the key genes controlling seed weight in major quanti-tative trait loci(QTL)is of great significance to improve soybean yield.In this study,a high-density genetic linkage map was con-structed by SLAF-seq using 325 recombinant inbred lines(RILs)constructed by Qihuang 34×Dongsheng 16 as the experimental materials.The total map distance was 2945.26 cM,and the average map distance was 0.47 cM.Combined with three environ-mental 100-seed weight phenotypes,11 QTL related to 100-seed weight were detected.Among them,the QTL with stable envi-ronment was qSW20-1,which explaining 9.73%–18.10%of phenotypic variation.The large grain allele of the QTL could signifi-cantly increase the number of seed per plant and seed weight per plant,but there was no adverse effect on the two quality traits of protein content and fat content.The interval size was 435.42 kb,containing 36 genes.Five candidate genes were predicted by gene annotation and expression pattern analysis.The results of this study laid a solid foundation for soybean yield-increasing gene mining and molecular design breeding.
作者
孙建强
洪慧龙
张勇
谷勇哲
高华伟
周雅
曹杰
祁航
赵权
包立高
陈庆山
邱丽娟
SUN Jian-Qiang;HONG Hui-Long;ZHANG Yong;GU Yong-Zhe;GAO Hua-Wei;ZHOU Ya;CAO Jie;QI Hang;ZHAO Quan;BAO Li-Gao;CHEN Qing-Shan;QIU Li-Juan(College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization,Ministry of Agriculture and Rural Affairs/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Keshan Branch of Heilongjiang Academy of Agricultural Sciences,Qiqihar 161606,Heilongjiang,China;Agriculture and Animal Husbandry Technology Promotion Center of Inner Mongolia Autonomous Region,Hohhot 010018,Inner Mongolia,China)
出处
《作物学报》
CAS
CSCD
北大核心
2023年第10期2621-2632,共12页
Acta Agronomica Sinica
基金
国家重点研发计划项目(2021YFD1201600)资助。
