摘要
目的通过网络药理学及设计空间法对乳腺康提取工艺参数进行研究。方法借助网络药理学对乳腺康潜在的活性成分进行筛选,并与酪氨酸激酶进行分子对接,结合《中国药典》2020年版确定指标性成分,采用HPLC法运用设计空间进行乳腺康提取工艺研究。结果筛选出乳腺康中甘草查尔酮A、川陈皮素、蒲公英甾醇等核心成分与酪氨酸激酶的亲和力与临床推荐用药相似;设计空间得到最佳提取工艺:浸泡时间为30 min、溶媒量为12倍、提取时间为45~75 min、乙醇体积分数为65%~80%、提取2~3次。结论实验所得到的工艺合理可行,验证实验与理论值预测值接近,具有一定的实用价值。该研究基于质量源于设计(QbD)理念的乳腺康提取工艺,稳定可靠,为其进一步的工艺开发及质量控制提供思路。
Objective To explore the extraction process parameters of Ruxiankang(乳腺康)through network pharmacology and design space.Methods With the help of network pharmacology,the active ingredients of Ruxiankang were screened,and molecular docking with tyrosine kinase was carried out,combined with"Chinese Pharmacopoeia"2020 edition to determine the index components,and the extraction process of Ruxiankang by high performance liquid chromatography and design space was studied.Results The core components of Ruxiankang screened,such as licochalcone A,nobiletin,and taraxasterol,had affinity with tyrosine kinases similar to those recommended in the clinic,and the optimal extraction process was obtained in the design space:the immersion time was 30 min,and the solvent volume was 12 times,extraction time was 45—75 min,ethanol concentration was 65%—80%,for 2—3 extraction times.Conclusion The process obtained in the experiment is reasonable and feasible,the verification experiment is close to the predicted value of the theoretical value,which has certain practical value.The Ruxiankang extraction process is based on the QbD concept,which is stable and reliable,and provides ideas for its further process development and quality control.
作者
刘博文
戴静
刘晓凤
闫博文
邓妍
刘涛
LIU Bo-wen;DAI Jing;LIU Xiao-feng;YAN Bo-wen;DENG Yan;LIU Tao(School of Pharmacy,Chengdu University,Chengdu 610106,China;Sichuan Tianyi University,Deyang 618000,China)
出处
《中草药》
CAS
CSCD
北大核心
2021年第6期1634-1644,共11页
Chinese Traditional and Herbal Drugs
基金
四川省科技成果转移项目(2020ZHCG0073)
2019年德阳市产学研合作科技研发类项目(2019CK090)
广西壮瑶药重点实验室2020年开放课题(GXZYKF202004)
成都大学CC国家众创空间2020年度创新创业项目(ccyg202001003)
四川省大学生创新创业训练计划项目(S202011079112X)。
