摘要
利用生物信息学方法,在西瓜测序基因组97103中共鉴定出96个bHLH家族成员,其中有94个可以被定位到西瓜的11条染色体上。通过基因结构和结构域序列保守性的预测,发现这些基因的序列长度和内含子数量变化很大,但bHLH结构域序列比较保守。用拟南芥中39条已知的bHLH蛋白序列和西瓜的96条bHLH蛋白序列构建系统发育树,结果显示西瓜的bHLH家族可以进一步被分为11个亚族。运用荧光定量实时PCR技术,分析了该家族中21个基因在西瓜响应非生物胁迫时的表达水平,结果表明,8个基因受低温胁迫诱导表达,13个基因受ABA胁迫诱导表达,14个基因受盐胁迫诱导表达。ClabHLH41在3种胁迫下表达量均显著增加,说明其在低温、ABA和盐胁迫应答中可能发挥着重要作用。
The bHLH family is a large superfamily of transcription factors（TFs）in plants,which play critical roles in plant development and response to diverse environmental stimuli. The analysis of evolution and structure of bHLH genes and their expression under abiotic stresses in watermelon conduced to a deep understanding of them and their application. Based on bioinformatics methods,a total of 96 bHLH genes were identified and 94 of them were mapped onto 11 chromosomes. Sequence analysis showed that bHLH genes were diverse both in length and numbers of intron,but were conserved in bHLH domain region. The phylogenetic tree,which was constructed with amino acid sequences of bHLH domain of 96 and 39 bHLHs from watermelon and Arabidopsis,indicated that bHLH family of watermelon could be further divided into 11 subfamilies. Using quantitative real-time PCR,expression levels of 21 bHLH genes were analyzed under three different abiotic treatments. Results showed that 8,13 and 14 genes could be induced to express by low temperature,ABA and salt stress,respectively. Importantly,gene ClabHLH41 had significantly higher expression levels under all three abiotic treatments,indicating its important function in response to these stresses in watermelon.
出处
《园艺学报》
CAS
CSCD
北大核心
2016年第2期281-294,共14页
Acta Horticulturae Sinica
基金
国家现代农业产业技术体系建设专项资金项目(CARS-26-18)
