摘要
从细叶百合(Lilium pumilum)中克隆得到LpSVP基因,该基因ORF全长为675bp,编码224个氨基酸。序列同源性和系统进化树分析表明该基因属于MADS-box基因家族下SVP类基因;生物信息学分析表明蛋白相对分子量为25.783kDa,理论等电点为5.62,为亲水蛋白,没有跨膜结构;亚细胞定位预测于细胞核中;蛋白质二级结构a-螺旋占60.71%,随机卷曲占29.02%,延伸链占10.27%;蛋白质三级结构以同型四聚体形式存在。半定量分析表明该基因在鳞茎、叶片、雌蕊、雄蕊和花瓣中均表达,但表达有强弱差异;实时荧光定量分析表明在花发育过程中该基因表达量先增长后下降。该研究为深入了解LpSVP基因功能奠定了基础。
The LpSVPgene was cloned fromLilium pumilum,the full-length of ORF was 675 bp,and encoding 224 amino acids.Sequence homology and phylogenetic analysis showed that this gene belonged to SVP-like MADS-box genes;bioinformatics analysis showed that the relative molecular weigh of the LpSVPgene was 25.783 kDa,the theoretical isoelectric point was 5.62,and it was hydrophilic protein with no transmembrane structure;the subcellular localization was predicted in nucleus;the secondary structure analysis showed that LpSVPcomprised 60.71% of alpha-helix,29.02%random coil and 10.27% extended strains;the tertiary structure of the LpSVPprotein existed as isomeric tetramer.RT-PCR analysis showed that the gene was expressed in bulb,leaf,pistil,stamen and petal,but varied widely in the level of expression.Real-time PCR analysis showed that the expression of LpSVPincreased firstly and then decreased continuously during flower development.The study would lay a foundation for further understanding the function of the gene.
作者
汪王
苏小霞
杨柳慧
周蕴薇
WANG Wang;SU Xiao-xia;YANG Liu-hui;ZHOU Yun-wei(College of Landscape Architecture,Northeast Forestry University,Ha thin,Heilongjiang 150000,China)
出处
《西北林学院学报》
CSCD
北大核心
2018年第4期89-94,共6页
Journal of Northwest Forestry University
基金
国家自然科学基金"低温解除细叶百合鳞茎休眠过程中的糖代谢动态及ADP核糖基化因子的生理功能"(31470698)
