摘要
【目的】探明白菜'矮脚黄'OguCMS核质互作的机制。【方法】采用cDNA-AFLP技术分析白菜'矮脚黄'OguCMS及其保持系花蕾基因的表达差异,获得一个在保持系早期花蕾不表达但在OguCMS早、中、晚花蕾中稳定表达的差异片段。利用RACE技术获得其cDNA全长,命名为BcBHLHogu(GenBank登录号:EF127860)。【结果】该基因编码122个氨基酸,具有basic helix-loop-helix(bHLH)结构域;进化分析表明,BcBHLHogu与拟南芥AtBHLH060同源性最高,是冗余基因AtBHLHs060/048的直系同源基因,其功能与BEEs1/2/3(3个油菜素内酯信号的早期应答冗余基因)相似。【结论】BcBHLHogu是白菜bHLH转录因子家族的一个新成员,涉及到花器官发育信号事件,其功能与油菜素内酯应答有关。
[Objective] This study was carried out in order to better understand the mechanism of nuclear-cytoplasmic interactions in OguCMS Chinese cabbage pak-choi （Brassica campestris L. ssp. chinensis Makino var. communis Tsen et Lee cv. Aijiaohuang）. [ Method ] Expression profiling was performed using cDNA-AFLP technology on floral buds of OguCMS lines and maintainers in Chinese cabbage pak-choi. A differentially expressed cDNA fragment was obtained, which was unexpressed in early-stage buds of maintainers, but expressed steably in OguCMS floral buds. The full cDNA, designated as BcBHLHogu （GenBank accession numbers： EF127860）, was obtained by RACE. [Result] It was found that BcBHLHogu coded 122 amino acids, contained a bHLH domain, and shared high homology with AtBHLH060. Phylogenetic analysis indicated that BcBHLHogu and AtBHLHs060/048 were orthologs genes. Its function is similar to BEEs1/2/3, which are three redundant brassinostreriod early response genes. [Conclusion] It is speculated that BcBHLHogu is a novel bHLH transcription factor in Chinese cabbage, and it appears to be involved in developmental signaling events for floral processes and has a function related to brassinosteriods signaling response.
出处
《中国农业科学》
CAS
CSCD
北大核心
2007年第8期1746-1752,共7页
Scientia Agricultura Sinica
基金
浙江省自然科学基金(Y304180)
浙江省重大科技项目(2005C12019-02)资助
关键词
白菜
bHLH转录因子
克隆
特征分析
Chinese cabbage （Brassica carnpestris L. ssp. Chinensis makino）
bHLH transcription factor
Cloning
Characterization
