摘要
建立灵敏、快速的反式阿魏酸（trans-FA）血浆浓度的高效液相色谱-串联质谱（HPLC-MS/MS）分析方法,研究反式阿魏酸在健康中国受试者体内的药动学特征。血浆样品经乙酸乙酯液-液萃取,采用Hedera ODS-2色谱柱分离,流动相为甲醇-5 mmol·L-1乙酸铵水溶液（含0.05%乙酸）（34∶66）,流速为0.4 m L·min-1;质谱采用Turbo-Ionspray电喷雾离子源和负离子多反应监测方式。反式阿魏酸在0.1-5 ng·m L-1内线性关系良好（r≥0.999 2）;方法的日间和日内精密度均小于9.2%,准确度为95.4%-111.4%;无基质效应,无残留效应;稳定性良好。所建立的HPLC-MS/MS分析方法快速、灵敏、准确、专属性强、重现性高,适用于健康中国受试者体内反式阿魏酸的药代动力学研究。
In this study, we developed a sensitive and rapid HPLC-MS/MS method for the determination of trans-ferulic acid（trans-FA） in plasma samples, and investigated the pharmacokinetics characteristics in healthy volunteers. The plasma samples were extracted with acetic ether, and then separated on a Hedera ODS-2 column with a mobile phase of methanol and 5 mmol·L-1 ammonium acetate buffer solution containing 0.05% acetic acid（34∶66） at a flow rate of 0.4 m L·min-1. Electrospray ionization source was applied and operated in the positive ion mode using MRM. The method exhibited a good linearity over the concentration range of 0.1-5 ng·m L-1（r ≥ 0.999 2）. The values on both the occasions（intra- and inter-day） were all within 9.2%, and the accuracy was 95.4%-111.4%. No matrix effect and carry-over effect were observed. Trans-FA was stable in human plasma under different storage conditions. The developed HPLC-MS/MS method is rapid, sensitive, accurate, and reproducible, and suitable for the pharmacokinetic study of trans-FA in healthy Chinese volunteers.
出处
《药学学报》
CAS
CSCD
北大核心
2016年第3期450-454,共5页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(81273482
81503169)
