摘要
目的建立白芷质量的高效液相色谱指纹图谱评价方法。方法以白芷药材为研究对象,应用RP-HPLC法,色谱柱为Krom asil C18(250 mm×4.6 mm,5μm),乙腈-水二元梯度洗脱模式,流速为1.0 mL.m in-1,检测波长为254 nm,建立了21批不同产地白芷样品的HPLC指纹图谱。结果对各产地药材指纹图谱进行聚类分析,根据聚类分析结果将白芷样品分为4类,选定其中11批优质样品建立共有模式,并应用“中药色谱指纹图谱相似度评价系统”对各产地药材进行了质量评价。结论该方法简便、可靠,可用于白芷的真伪鉴别和质量评价。
Aim To establish the RP-HPLC fingerprint analysis for the quality control of Radix Angelicae dahuricae. Methods HPLC fingerprint analysis method of Radix Angelicae dahuricae was developed. Kromasil Cjs column （250 mm×4.6 mm ID, 5μm） was used, with mixture of acetonitrile and water as mobile phase in a gradient mode. The flow rate was 1.0 mL·min^-1 The wavelength of measurement was 254nm. Twenty-one batches of Radix Angelicae dahuricae were determined. Results The 21 samples were classified as 4 clusters by cluster analysis and the 11 superior in producing area samples were confirmed to establish the mutual model. The samples＇ quality was assessed by Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004. Conclusion The method can be used to identify and evaluate the quality of Radix Angelicae dahuricae conveniently.
出处
《药学学报》
CAS
CSCD
北大核心
2006年第8期747-751,共5页
Acta Pharmaceutica Sinica
关键词
白芷
指纹图谱
反相高效液相色谱法
Radix Angelicae dahuricae
chromatographic fingerprint
RP-HPLC
