摘要
2011年海南会文烟堆某豹纹鳃棘鲈(Plectropomus leopardus)养殖场突发大量死亡,病鱼体色发白,尾鳍溃烂。从病鱼病灶处分离出1株菌X11YD05,回归感染证实为患病豹纹鳃棘鲈病原菌,对平均体质量为30.8 g的斜带石斑鱼(Epinephelus coioides)的半数致死量(LD50)为1.67×105cfu·g-1。为进一步确定病原菌的分类学特征,扩增其16S rDNA、toxR、rpoD、mreB、topA和rctB等6种管家基因,构建系统发育树。结果发现,X11YD05与哈维弧菌(Vibrio harveyi)有较高同源率。结合生理生化试验,确定X11YD05为哈维弧菌。16种药敏试验表明该菌仅对氧氟沙星、诺氟沙星、环丙沙星、恩诺沙星、复方新诺明等5种抗生素敏感。
In 2011, there was an outbreak in a Plectropomus leopardus farm in Wenhui Town, Yandui Village, Hainan Province. The color of sick fish turned white and the tail fin festered. We isolated a Strain X11YD05 from sick P. leopardus which was proved to be pathogen by artificial infection and the LDs0 was 1.67 × 10^5cfu.g-1 in Epinephelus coioides using Karber method. To determine the molecular characteristics of X1 t YD05, we amplified the 16S rDNA, transmembrane regulatory protein （toxR）, RNA polymerase 570 factor （ rpoD）, actin-like cytoskeleton protein （ mreB）, topoisomerase （topA） and replication origin-binding protein （rctB） by PCR. Sequence analysis indicates that X11YD05 belonged to V. harveyi. Drug sensitivity test shows that X11YD05 was sensitive to norfloxa- cin, ofloxacin, ciprofloxacin, enrofloxacin and cotrimoxazole.
出处
《南方水产科学》
CAS
CSCD
北大核心
2015年第4期71-80,共10页
South China Fisheries Science
基金
国家自然科学青年基金项目(31202027)
中国水产科学研究院院级基本科研业务费项目(2014KX0604)
海南省科技兴海项目(2015XH03)
