摘要
目的探讨慢性不可预见性应激(chronic unpredictable stress,CUS)对大鼠神经递质和下丘脑-垂体-肾上腺皮质轴(hypothalamic-pituitary-adrenal axis,HPA)的影响。方法 16只SD雄性大鼠随机数字表法分为正常对照组和模型组。模型组大鼠给予慢性不可预见性的应激,检测其体质量增长量;通过蔗糖偏嗜实验观察大鼠行为学的改变;采用高效液相-电化学法测定海马多巴胺(dopamine,DA)、5-羟色胺(5-hydroxytryptamine,5-HT),高效液相-荧光法测定谷氨酸(glutamic acid,Glu)、γ-氨基丁酸(γ-aminobutyric acid,γ-GABA);用放射免疫法检测血浆及下丘脑促肾上腺皮质激素释放激素(corticotropin-releasing hormone,CRH)、促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)、皮质酮(corticosterone,CORT)。结果与正常对照组相比,模型组大鼠体质量增长量显著降低,糖水消耗量明显下降,DA、γ-GABA显著降低,5-HT、Glu有升高的趋势,ACTH显著降低,CORT质量浓度明显增高。结论 CUS会导致大鼠行为学的改变,HPA轴的紊乱。
Objective To investigate the effect of chronic unpredictable stress（ CUS） on neurotransmitter and hypothalamic-pituitaryadrenal axis（ HPA） in rats. Methods Totally 16 male SD rats were randomly divided into normal group and model group. The model group was established by chronic unpredictable stress. The weights of rats were examined every day. The behavior changes of rats were observed by the sucrose preference test. The contents of dopamine（ DA）,5-hydroxeytryptamine（ 5-HT） inhippocampus were measured by High performance liquid-electrochemical method. And the secretion of glutamic acid（ Glu）,γ-aminobutyric acid（ γ-GABA） in hippocampus were determined by the method of high-effective liquid chromatography fluorescence. The concentrations of corticotropinreleasing hormone（ CRH）,adrenocorticotropic hormone（ ACTH）,corticosterone（ CORT） in plasma and hypothalamus were tested by Radioimmunoassay（ RIA） method. Results Compared with the normal group,the model group＇s weight was significantly reduced. The consumption of sucrose was decreased in model group. The contents of DA,γ-GABA in hippocampus was significantly reduced in model group,and the 5-HT,Glu had the trend of increase. The adrenocorticotropic hormone（ ACTH） in hypothalamus was significantly decreased and the plasma＇s corticosterone（ CORT） was significantly increased in model group. Conclusion The chronic unpredictable stress（ CUS）could induce the changes of animal behavior and abnormalities of neurotransmitter and hypothalamic pituitary adrenal（ HPA） axis.
出处
《首都医科大学学报》
CAS
北大核心
2015年第1期127-131,共5页
Journal of Capital Medical University
基金
北京市教委基金(KM201310025013)
北京市属高等学校高层次人才引进与培养计划-长城学者项目资助(CIT&TCD20140329)~~
