摘要
目的 探讨c myc基因在原发性肝细胞癌中的扩增及其与临床及预后的关系。方法 应用间期核荧光原位杂交技术对 10 0例原发性肝癌和 6例带卫星结节的肝癌组织标本进行检测。结果 92 % (92例 )原发性肝癌组织存在c myc基因扩增 ,其中高拷贝数扩增达 37% (37例 ) ,低拷贝数扩增 5 5 % (5 5例 ) ,只有 8% (8例 )无扩增。结合临床资料分析 ,c myc扩增与患者临床分期、肿瘤大小和病理类型无明显相关 (P >0 .0 5 ) ,原发肿瘤和相应卫星结节肿瘤组织具有相似的c myc基因扩增水平和相同病理类型的特点。在可随诊两年半的 90例病例中 ,91.1% (82例 )病例有c myc基因扩增 ,其中高拷贝数扩增 34例 ,低拷贝数扩增 48例 ,无扩增 8例。分析c myc扩增程度与患者术后复发的时间及生存期关系 ,发现c myc高拷贝数扩增的病例术后 1年内复发率 (70 .6 % ,2 4/ 34 )高于c myc低拷贝数扩增 (39 6 % ,19/ 48)和无扩增者 (12 5 % ,1/ 8) ,三者之间差异有显著性意义 (P <0 .0 5 )。而在术后 >12个月的复发率则与c myc不同扩增程度间差异无显著性意义 (P >0 .0 5 )。另外 ,c myc高拷贝数扩增者术后 2年存活率明显低于低拷贝数扩增和无扩增者 (P =0 .0 0 1)。结论 原发性肝细胞癌存在高频率的c myc基因扩增 ,基因扩增与患者临床?
Objective To investigate the relationships between c-myc oncogene amplification in primary hepatocellular carcinoma (HCC) and its clinical prognosis. Methods To detect c-myc oncogene amplification in 100 cases with primary HCC and in 6 cases with satellite nodes by interphase fluorescence in situ hybridization (FISH). Results c-myc amplification was detected in 92 of 100 (92%) cases with HCC, 37 of which (37%) showed high copy amplification (HC), 55 (55%) low copy amplification (LC) and 8 (8%) single copy or no amplification (SC). There wasn′t statistically different significance between the frequency of c-myc amplification and clinical stage and tumor size, and pathological types (P>0.05), however, in the 90 follow-up resected cases, the one-year rates of recurrence in HC, LC and SC were 70.6% (24/34), 39.6% (19/48) and 12.5% (1/8) respectively (P<0.05) but the recurrence rates after more than one year were 14.70% (5/34), 14.6% (7/48) and 0 (0/8) respectively (P>0.05). Meanwhile, two-year survival rate of patients with HC was significantly lower in comparsion with those with LC and SC (P=0.001). Conclusions There is a high frequency of c-myc oncogene amplification in primary HCC and the fact that the patients with HC showed poorer prognosis indicates that the level of c-myc oncogene amplification may be a sensitive index in the prognosis of HCC.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2001年第3期180-182,共3页
Chinese Journal of Pathology
基金
国家杰出青年B类基金资助项目 (3 982 5 5 11)
广东省自然科学基金资助项目 (980 10 7)
关键词
肝细胞癌
荧光原位杂交
MYC基因
预后
基因扩增
Liver neoplasms
Carcinoma, hepatocellular
In situ hybridization, fluorescence
Genes, myc
Prognosis
