摘要
目的探讨RA患者PBMCs上HLA-G的表达及其与RA疾病活动度、病程、临床用药及各实验室指标的相关关系。方法采用流式细胞术检测68例RA患者和63名健康对照者PBMCs中淋巴细胞、CD3＋T细胞和CD14＋单核细胞上HLA—G的表达。采用非参数Mann-Whitnev U检验、Speatinan等级相关分析。结果RA患者外周血HLA—G＋淋巴细胞百分比显著低于健康对照组[1．98％（1．17％～3．66％与2．93％（1．70％-5．40％），U=1624．5，P〈0．05]，而且HLA—G＋淋巴细胞百分比与RA疾病活动度DAS28评分呈负相关（r=-0．288，P=0．017）；CD3＋HLA-G＋T细胞百分比亦明显降低[1．35％（0．57％～2．79％）与2．16％（1．15％～4．45％），U=1387，P=-0．000]，且与DAS28评分呈负相关（r—0．373，P=0．001）；CDl=14＋HLA—G＋单核细胞百分比明显低于健康对照组[22．65％（10．59％～36．81％）与32．76％（20．73％～44．62％），U=I526，P〈0．01]，但未发现CD14＋HLA-G单细胞百分比与DAS28评分存在显著相关性（P〉O．05）。此外，上述各类细胞上HLA—G的表达与RA患者病程、临床用药及各实验室指标如RF、ESR、CRP、抗CCP抗体和ANA水平等均无显著相关性（均P〉0．05）。结论RA患者外周血淋巴细胞、CD3＋T细胞和CD14＋单核细胞上HLA．G的表达异常降低，HLA—G分子的异常降低可能导致机体免疫抑制功能低下，从而促进了RA的发生发展。
Objective To investigate the expression of HLA-G in lymphocytes, CD3＋T cells, and CD14＋ monocytes from patients with rheumatoid arthritis （RA） and their associations with disease activity, disease duration, medication used and clinical parameters. Methods We studied 68 RA patients as well as 63 healthy controls. HLA-G expression was analyzed by flow cytometry in peripheral blood mononuclear ceils （PBMCs）. Mann Whitney U tests and Spearman rank correlation coefficient test were used for statistical analysis. Results We found that lymphocytes as well as CD3＋T cells, and CD14＋monocytes in RA patients showed a diminished expression of HLA-G compared with healthy controls [ 1.98%（1.17%-3.66%） vs 2.93% （1.70%-5.40%）, U=1624.5, P〈0.05; 1.35%（0.57%-2.79%） vs 2.16% （1.15%-4.45%）, U=1387, P=0.000; 22.65% （10.59%-36.81%） vs 32.76% （20.73%-44.62%）, U=1526, P〈0.01 ]. Spearman analysis showed that the levels of HLA-G~ lymphocytes as well as CD3＋ HLA-G~ T cells was negatively associated with DAS28 score（r=-0.288, P= 0.017; r=-0.373, P=0.001）, but no significant correlation was detected between the levels of CD14~HLA-G＋monocytes and DAS 28 score （/9〉0.05）. In addition, the levels of expression of HLA-G was not correlated to disease duration, medication used, ESR, CRP, RF, anti-CCP and ANA （P〉0.05）. Condttsion Decreased expression of HLA-G are detected in lymphocytes, CD3＋ T cells, and CD14＋ monocytes from pa-tients with RA, and the abnormality of HLA-G may play an important role in the development of RA.
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2014年第5期325-328,共4页
Chinese Journal of Rheumatology
基金
浙江省自然科学基金（LY12H10002）
浙江省科技计划（2010C33001）
