摘要
目的探讨程序性细胞死亡因子5(PDCD5)在喉鳞状细胞癌组织中的表达及与临床病理指标的相关性。方法采用逆转录多聚酶链式反应(RT-PCR)技术和Western blot免疫印记技术检测41例喉鳞状细胞癌及29例癌旁正常黏膜组织中PDCD5mRNA和蛋白的表达情况,进一步应用免疫组化方法验证其在喉鳞状细胞癌组织中的定位表达。结果 PDCD5 mRNA喉鳞状细胞癌组织中较癌旁正常组织的表达明显降低,表达差异有统计学意义(P=0.006);Western blot结果显示PDCD5蛋白在喉鳞状细胞癌组织中较癌旁正常组织的表达明显降低,表达差异有统计学意义(P=0.012)。肿瘤组织中PDCD5 mRNA表达缺失与PDCD5蛋白表达缺失之间存在明显的相关性(Pearson r=0.695,P<0.01)。免疫组化进一步证实PDCD5在肿瘤组织中的高频率缺失。χ2检验分析发现PDCD5蛋白表达缺失与喉鳞状细胞癌患者的年龄、性别、肿瘤原发部位和有无淋巴结转移无相关性,但是与肿瘤TNM分期以及组织学分级之间相关(mRNA P=0.032,P=0.021;蛋白P=0.027,P=0.016)。结论 PDCD5在喉癌中多呈低表达,在喉鳞状细胞癌的发生、发展过程中可能起重要作用。
Objective To determine expression of programmed cell death 5（PDCD5） in laryngeal squamous cell carcinoma and reveal its possible correlation to clinical parameters.Methods Expression of mRNA and protein of PDCD 5 were analysed in 41 laryngeal squamous cell carcinoma and 29 normal tissues adjacent to the carcinomas with reverse transcription-polymerase chain reaction（RT-PCR） and Western blot analysis.Immunohistochemistry was also applied to investigate protein expressions of PDCD 5 in squamous cell carcinoma and matched normal tissues.Result The normal tissues adjacent to tumor lesions expressed higher levels of PDCD5 mRNA,whereas most of laryngeal squamous cell carcinoma expression was reduced or did not expres（P=0.006）.Compared with the normal tissues,most carcinoma exhibited less PDCD5 protein（P=0.012）.By immunohistochemistry,the loss of PDCD5 expression was identified as a frequent event in laryngeal squamous cell carcinoma.Expressions of PDCD5 protein and mRNA were correlated with clinical stages and histological gradings.There was no correlation with age,gender,tumor site or lymph node metastasis.Conclusions The decrease or absence of PDCD5 expression is a frequent event in laryngeal squamous cell carcinoma,which might contribute to tumor development and progression.
出处
《山东大学耳鼻喉眼学报》
CAS
2012年第2期23-26,31,共5页
Journal of Otolaryngology and Ophthalmology of Shandong University
