摘要
采用酶法提取平菇菌丝体多糖,通过L9(34)正交试验考察加酶量、温度、时间、pH值对平菇菌丝体多糖提取率的影响,确定其最佳提取条件;通过DEAE-32离子交换层析和Sephadex G100凝胶过滤纯化平菇菌丝体多糖,DPPH自由基清除作用测定抗氧化活性,紫外光谱扫描和薄膜电泳方法鉴定多糖的纯度;利用化学比色法检测其对O2-·和·OH的清除能力和对小鼠红细胞溶血抑制程度。结果表明:平菇菌丝体多糖最佳提取条件为加酶量1.0%、温度55℃、时间2h、pH5.3,最佳条件下多糖提取率为(4.91±0.15)%;纯化后的多糖能清除O2-·和·OH及抑制红细胞溶血的生成,且呈现一定剂量关系。表明平菇菌丝体多糖在一定浓度范围内具有体外抗氧化作用。
Crude polysaccharides were extracted from Pleurotus ostreatus mycelium by cellulase. Optimal extraction conditions were explored to evaluate the effects of enzyme dosage, extraction temperature, extraction time and pH on extraction rate of polysaccharides through orthogonal experiments. Crude polysaccharides were purified with DEAE-32 and Sephadex G100 column chromatography. The purity of polysaccharides from Pleurotus ostreatus mycelium was determined by UV scanning and cellulose acetate electrophoresis. Antioxidant activity of polysccharides was evaluated by the scavenging capability of surperoxide anion and hydroxyl free radicals and the inhibition rate of erythrocyte hemolysis. Results indicated that the optimal extraction conditions for preparing polysaccharides from Pleurotus ostreatus mycelium by cellulase were enzyme dosage of 1.0%, extraction temperature of 55 ℃, extraction time of 2 h and pH 5.3. The extraction rate was up to （4.91 ±- 0.15）% under the optimal extraction conditions. The purified polysaccharides from Pleurotus ostreatus mycelium revealed antioxidant activity such as scavenging capability of free radicals and suppression of erythrocyte hemolysis in a dosage-dependent manner. Therefore, the polysaccharides from Pleurotus ostreatus mycelium have strong antioxidant activity in vitro in a certain concentration range.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第22期124-128,共5页
Food Science
基金
辽宁省教育厅高等学校科学技术研究项目(2009A811
L20100154)
辽宁省科技厅农业攻关计划项目(2009209001)
辽宁大学"211工程"三期建设项目
关键词
平菇菌丝体
多糖
纯化
抗氧化
Pleurotus ostreatus mycelium
polysaccharides
purification
antioxidation
