摘要
目的探讨铝对新生大鼠海马细胞内游离钙浓度([Ca2+]i)的影响。方法采用体外实验的方法,将分离的新生Wistar大鼠海马细胞制成2×106个/ml的细胞悬液,经Fura-2-AM负载后,与氯化铝溶液(Al3+终浓度分别为0、10、100、1000μmol/L)一起孵育,分别测定孵育15、30、45min后海马细胞悬液的荧光强度值(F)、最大荧光强度值(Fmax)、最小荧光强度值(Fmin),并计算海马神经细胞内[Ca2+]i。结果孵育15、30和45min后,1000μmol/LAl3+组海马细胞中[Ca2+]i均高于对照组(0μmol/LAl3+组),差异有统计学意义(P<0.05)。而10、100μmol/LAl3+组[Ca2+]i与对照组比较,差异均无统计学意义(P>0.05)。结论铝可通过增加新生大鼠海马神经细胞内[Ca2+]i而发挥神经毒性作用。
Objective To explore the effect of aluminium on the concentration of intracellular Ca^2＋ in the hippocampus neurons of postnatal rat. Methods The hippocampus neurons of postnatal rats were primarily cultured and treated with Al^3＋ at doses of 0, 10, 100 and 1 000 μmol/L. Fura-2-AM calcium ions fluorescence indicator was used to determine the concentration of the intracellular Ca^2＋ when they were cultured for 15min, 30min and 45 min. Results The concentration of intracellular Ca^2＋ in the hippocampus neurons increased with the aluminum concentration increased and as the concentration of the aluminium was 1 000 μmol/L, at the three cultured time points, the concentration of intracellular Ca^2＋ were （276.2±53.4） nmol/L, （306.5 ±73.7） nmol/L and （408.8 ±79.7） nmol/L respectively, significantly higher than the control group （P〈0.05）. Conclusion Al^3＋ may produce the neurotoxic effect by increasing the concentration of intracellular Ca^2＋ in the hippocampus neurons.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2007年第7期527-528,共2页
Journal of Environment and Health
关键词
铝
钙
海马
Aluminium
Calcium
Hippocampus
