摘要
利用哺乳动物线粒体基因的保守序列设计引物,采用PCR方法,首次从亚洲黑熊四川亚种(Ursusthibetanus mupinensis)的肌肉组织总DNA中扩增出了线粒体12S rRNA和16S rRNA基因并进行了序列测定及分析.结果表明,四川黑熊12S rRNA基因长965 bp;16S rRNA基因长1 580 bp.通过进一步的序列分析表明,四川黑熊的12S rRNA和16S rRNA基因有较高的进化速率,与美洲黑熊、棕熊、北极熊、眼镜熊及大熊猫的相应基因相比较有较大差异,其中与美洲黑熊的同源性相对较高.
By means of the primer designed according to the conservative sequence of mammalian mitochondrial gene, mitochondrial 12S rRNA and 16S rRNA gene were amplified from the Asian black bear Sichuan subspecies＇ muscle tissue total DNA by PCR method. Their sequences were determined and analyzed. The result indicated that 12S rRNA gene length and 16S rRNA gene length of Sichuan black bear were 965 bp and 1 580 bp respectively. The further sequence analysis showed that 12S rRNA and 16S rRNA gene of Sichuan black bear had a higher evolution rate, which had more differences than that of American black bear, brown bear, polar bear, spectacled bear and panda, but Sichuan black bear had more homology with American black bear.
出处
《四川师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第3期402-405,共4页
Journal of Sichuan Normal University（Natural Science）
基金
国家自然科学基金(30570275)
四川省应用技术基金
四川省教育厅自然科学重点基金
四川省重点学科建设基金资助项目
