摘要
为进一步研究甘薯病原微生物的侵染对甘薯病程相关非表达子1基因(NPR1)表达的影响,以甘薯肌动蛋白(β-ac-tin)基因为内参照基因,提取甘薯叶片总RNA,反转录为cDNA,同批异管对该2个基因进行PCR扩增.通过对循环数的优化和对体系重复性、准确性的分析,建立了一个稳定、方便的半定量RT-PCR体系.
With β -actin gene as the inner control, the total RNA of sweet potato young leaves was extracted and reversely transcribed to form cDNA to further study the expression of nonexpresser of pathogenesis-related genes 1 （ NPR1 ） gene in Sweet potato suffering from pathogen stress. The fragments of the two genes were PCR amplified with the same cycle numbers during the exponential phase of NPR1 and β-actin in different tubes. Cycle number of PCR system was optimized and analyzed in their repeatability and precision, and then a stable and convenient semi-quantitative RT-PCR system was established. This system can be used to analyze the relationship between expressions of NPR1 gene in different sweet potato and pathogen, then to ascertain the expression pattern of NPR1 gene and function in sweet potato, to provide reference for the breeding of sweet potato resistant to disease.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2007年第1期56-59,共4页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省自然科学基金资助项目(2006J0059)
福建省教育厅重点项目(JA06011)
