摘要
水杨酸介导的植物系统获得性抗性的正向关键调控基因NPR1功能上在许多植物中极其保守。本研究利用生物信息学相关软件对以甘薯NPR1基因为主要分析对象的7个NPR1基因的核苷酸序列及其相应氨基酸序列的组成成分、氨基酸翻译后修饰、导肽、跨膜结构域、疏水性/亲水性、蛋白质二、三级结构以及功能结构域等进行分析。结果表明:该类基因属于不具信号肽的疏水性蛋白,α-螺旋和无规则卷曲是蛋白质二级结构最大量的结构元件,β-转角和延伸链散布于整个蛋白质中,具有BTB/POZ和ANK功能结构域。这一结果可为植物NPR1的结构与功能分析及利用研究提供进一步的信息与参考。
The. key regulator of salicylic acid（SA）-mediated systemic acquired resistance（SAR）, NPRl（nonexpressor of pathogenesis-related genes 1）is functionally conserved in diverse plant species. In the present study, 7 NPR1 genes from different plants, which were registered in GenBank, were analyzed and predicted by the tools of bioinformatics in the following aspects： the composition of nuclei acid sequences and amino acid sequences, signal peptide, transmembrane structure, hydrophobicity or hydrophilicity, secondary and tertiary structure of protein , molecular phylogenetic evolution and so on. The results are as follows： NPR1 is a hydrophobicity protein without a signal peptide; The main motif of the predicted secondary structure of NPR1 are alpha helix and random coil, beta turn and extended strand are scattered in the whole secondary structure of protein; The NPR1 proteins have BTB/POZ and ANK function domains. The work would be of great information and values for the further structure and function study and utilization of plant NPR1.
出处
《热带作物学报》
CSCD
2011年第5期881-885,共5页
Chinese Journal of Tropical Crops
基金
国家自然科学基金(30900915)
福建省自然科学基金项目(2010J05046)
