摘要
柠檬酸合成酶(Citrate Synthase,CS)是茶树体内合成柠檬酸的关键性酶。克隆了茶树根系柠檬酸合成酶基因片段,GenBank登录号为FJ814766,且利用半定量RT-PCR方法,研究该基因在低磷下的表达变化。结果表明,当供磷浓度为0(缺磷)时,根系CS基因的表达略有增强,这可能是茶树对低磷的一种适应机制。
Citrate synthase(CS) is the key enzyme of producing citrate acid. CS gene fragments were cloned from tea roots. The GenBank accession is FJ814766 in the NCBI. Then the changes in gene expression in response to P supply were investigated by using semi-quantitative RT-PCR. The result showed that P deficiency increased the expressions of CS gene. This may be a tolerant mechanism of tea seedlings to P deficiency.
出处
《茶叶科学》
CAS
CSCD
北大核心
2010年第5期362-366,共5页
Journal of Tea Science
基金
福建省自然科学基金(2009J01083)
科技部支撑计划(2007BAD07B02)
国家茶叶产业技术体系(nycytx-23)
福建省农科院创新团队"特色园艺作物育种与技术创新"
