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宏基因组文库技术获得聚酮化合物 被引量:12

Polyketides Obtained Using Metagenome Library Technique
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摘要 聚酮化合物是一类重要的具有生物活性的次级代谢物。由于运用传统方法从自然界中直接筛选新型天然聚酮化合物的重现率很高,近年来出现了很多开发新型聚酮化合物的新方法,文章主要介绍通过环境宏基因组文库获得新聚酮类化合物的方法。 Polyketides are an important class of bioactive secondary metabolites. Since the recurrence of the known polyketides is very high during the discovery of novel natural polyketide compounds using traditional methods, new approaches are developed for the biosynthesis of these compounds,. This review discusses discovery of new polyketides via metagenome library technique.
作者 杨建 洪葵
出处 《遗传》 CAS CSCD 北大核心 2006年第10期1330-1336,共7页 Hereditas(Beijing)
基金 国家自然科学基金(编号:30560005) 科技部社会公益项目(编号:004DIB3J072)资助~~
关键词 聚酮化合物 宏基因组文库 polyketide metagenome library
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参考文献53

  • 1Weissman K J. Polyketide biosynthesis: understanding and exploiting modularity. Philos Transact A Math Phys Eng Sci, 2004,362(1825): 2671-2690.
  • 2Rosello-Mora R, Amann R. The species concept for prokaryotes.FEMS Microbiol Rev, 2001, 25(1): 39-67.
  • 3Amann R I, Ludwig W, Schleifcr K H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation.Microbiol Rev, 1995, 59(1): 143-169.
  • 4Handelsman J, Rondon M R, Brady S F, Clardy J, Goodman R M. Molecular biological accesses to the chemistry of unknown soil microbes: A new frontier for natural p products. Chem Biol, 1998,5(10): 245-249.
  • 5Wang G Y, Graziani E, Waters B, Pan W, Li X, McDermott J,Meurer G, Saxena G, Andersen R J, Davies J. Novel natural products from soil DNA libraries in a streptomyeete host. Org Lett, 2000, 2(16): 2401-2404.
  • 6Piel J A. Polyketide synthase-peptide synthetase gene cluster from an uncultured bacterial symbiont of Paederus beetles USA.a long-term fertilizer field experiment. Proc Natl Acad Sci USA, 2002, 99 (22): 14002-14007.
  • 7Courtois S, Cappellano C M, Ball M, Francou F X, Normand P,Helynck G, Martinez A, Kolvek S J, Hopke J, Osbume M S, August P R, Nalin R, Guerineau M, Jeannin P, Simonet P, Pernodet J L. Recombinant environmental libraries provide access to microbial diversity for drug discovery from natural products. Applied and Environmental Microbiology, 2003, 69(1): 49-55.
  • 8Shen B. Polyketide biosynthesis beyond the type Ⅰ, Ⅱ and Ⅲ polyketide synthase paradigms. Curt Opion Chem Biol, 2003, 7:285-295.
  • 9Pfelfer B A, Khosla C. Biosynthesis of polyketides in heterologous hosts. Microbiol Mol Biol Rev, 2001, 65 (1): 106-118.
  • 10Sherman D H, Malpartida F, Bibb M J, Kieser H M, Bibb M J,Hopwood D A. Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of Streptomyces.EMBO J, 1989, 8(9): 2717-2725.

二级参考文献21

  • 1Dunbar J, Ticknor L O, Kuske C R. Phylogenetic specificity and reproducibility and new method for analysis of terminal restriction fragment profiles of 16S rRNA genes from bacterial communities. Applied and Environmental Microbiology, 2001,67( 1):190 ~ 197
  • 2Lukow T, Dunfield P F, Leisack W. Use of the T-RFLP technique to assess spatial and temporal changes in the bacterial community structure within an agricultural soil planted with transgenic and non-transgenic potato plants. FEMS Microbial Ecology, 2000, 32:241 ~ 247
  • 3Muyzer G, Ellen C W, Andre G U. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction genes coding for 16S rRNA. Applied and Environmental Microbiology, 1993, 59: 695 ~ 700
  • 4Robe P, Nalin R, Capellano C, et al. Extraction of DNA from soil.European Journal of Soil Biology,2003, 39:183 ~ 190
  • 5Alm D W, Zheng D, Raskin L. The presence of humic substances and DNA in RNA extracts affects hybridization results. Applied and Environmental Microbiology, 2000, 66:4 547 ~ 4 557
  • 6Kresk M, Wellington E M. Comparison of different methods for the isolation and purification of total community DNA from soil. Journal of Microbiological Methods, 1999, 39:1 ~ 16
  • 7Robert I G, Andrew S W, Anthony G O. Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA- based microbial community composition. Applied and Environmental Microbiology, 2000, 66(12) :5 488 ~ 5 491
  • 8Gelsomine A, Keijzer-Woltem A C, Cacco G, et al. Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis. Journal of Microbiological Methods, 1999, 38(1): 1 ~ 15
  • 9ZhouJ, BrounsMA, Tiedje J M. DNA recovery from soils of diverse composition. Applied and Environmental Microbiology, 1996,62(2) :316 ~ 322
  • 10Borneman J, Skrotch PW, Palus JA, et al. Molecular microbial divemity of an agricultural soil in Wisconsin. Applied and Environmental Microbiology, 1996, 62(6):1 935 ~ 1 943

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