摘要
从丝瓜根部分离出来的桔抗菌ＴＧ２６，根据形态特征和生理生化特性，鉴定为枯草芽抱杆菌（Ｂａｃｉｌｌｕｓｓｕｂｔｉｌｉｓ）。该菌株能分泌大量的抗菌蛋白，经ＳｅｐｈａｄｅｘＧ－１５０柱和ＦＰＬＣＭｏｎｏＱ柱层析后得到单一组分的抗菌蛋白，命名为ＢＩ。ＢＩ能抑制多种植物病原菌的生长，对热稳定，对蛋白酶部分敏感。经ＳＤＳ－ＰＡＧＥ和等电聚焦电泳测定其分子量约１４．５ｋＤ，等电点为５．５８。氨基酸组成分析表明，该蛋白宫含谷氨酸、酪氨酸和脯氨酸；并测定了Ｎ末端氨基酸的部分序列。
Antagonistic bacterium strain TG26 secreting large amount of antifungal proteins wasidentified to be Bacillus subtilis by its cell morphological, physiological and biochemical characters. One of the antifungal proteins from TG26 strain was separated by gel filtration onSephadex G-150 column and further purified with Mono Q column on FPLC. The purifiedprotein with a molecular weight of 14. 5 kD and a isoelectric point of 5' 58 inhibits stronglymycelial spread of many kinds of plant pathogens. A amino acid analysis of the protein BIshowed that glut aminic acid was the most abundant amino acid. No arginine. histidine.phenylalanine and tryptophane were found. The partial sequence of N-terminal was determined by Edman degradation.
基金
国家自然科学基金
关键词
拮抗菌
抗菌蛋白
提纯
转基因抗病
Antagonistic bacterium
Antifungal protein: Purification
