摘要
在pH 7.0的Britton-Robinson(B-R)缓冲溶液中用电化学分析方法研究了孔雀石绿(MG)与鲱鱼精ds-DNA的相互作用。循环伏安法表明,MG在-1.0^+1.5 V(vs.SCE)范围内有3个氧化还原峰。加入双链DNA后,由于MG与DNA相互作用,使MG在+0.547 V处的氧化峰电流明显降低,而峰电位基本没有改变。优化了结合反应条件,在最佳条件下,MG氧化峰电流的下降值同DNA的浓度在10.0~100.0 mg.L-1范围内呈良好线性关系,线性关系方程为Δip(μA)=0.066+0.009 6c(mg.L-1),检测限(3σ)为6.0 mg.L-1。通过DNA加入前后峰电流的变化,可计算出MG与DNA结合比n(MG)∶n(DNA)=2∶1,结合常数为5.67×108。
The interaction of malachite green （MG） with Herring Sperm double-stranded DNA （ds-DNA） in a Britton-Robinson （B-R） buffer solution of pH=7.0 was investigated by electrochemical method. The result of cyclic voltammetry showed that malachite green had three redox peaks from - 1.0 V to ＋ 1.5 V（vs. SCE）. The oxidative peak current at ＋0. 547 V was decreased after the addition of double-stranded DNA （dsDNA） whereas without the movement of peak potential. The decrease of the peak current was directly proportional to DNA concentration in the range of 10.0-100.0 mg· L^-1. The regression equation was △ip（μA）=0. 066＋0. 009 6c（mg · L^-1） and the detection limit was 6.0 mg · L^-1 （3σ）. It was calculated that the binding ratio of MG ： DNA was 2 ： 1 and the binding constant was 5.67× 10^8.
出处
《青岛科技大学学报（自然科学版）》
CAS
2006年第2期95-98,共4页
Journal of Qingdao University of Science and Technology:Natural Science Edition
基金
国家自然科学基金项目(20375020
20405008)
青岛市自然科学基金项目(04-2-JZP-8
04-2-JZ-114)
关键词
孔雀石绿
DNA
电化学分析
malachite green
DNA
electrochemical determination
