摘要
本文用荧光光谱法和紫外-可见吸收光谱法研究了肼黄与牛血清白蛋白(BSA)结合反应的光谱行为,发现肼黄因对BSA有较强的荧光猝灭作用,且肼黄的紫外吸收光谱和BSA的荧光发射光谱有一定程度的重叠,据此得出了其结合反应的结合常数、结合位点数和基本热力学参数等,并研究了二者的相互作用机理。
The binding reaction between Tartrazine and bovine serum albumin （BSA） was studied by using fluorescence spectra and UV absorption spectra. It is shown that this compound has a powerful abihty to quench the fluorescence intensity of BSA. The fluorescence quenching data were analyzed according to Stem-Volmer equation. The binding constant and the thermodynamic parameters were obtained. The interaction mechanism was also studied.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2006年第11期1297-1301,共5页
Chemical Research and Application
基金
山东省教育厅科技计划项目(j05B56)资助
关键词
肼黄
BSA
荧光光谱
紫外吸收光谱
Thebaine, bovine serum albumin
fluorescence spectrum
absorption spectrum
thermodynflmieparameters
