摘要
根据已发表的其他脊椎动物β-珠蛋白基因序列设计并合成1对特异性引物，通过RT-PCR方法首次克隆到美国红鱼和大黄鱼β-珠蛋白cDNA，其开放读码框均由447个碱基组成，编码148个氨基酸的多肽，预测的相对分子量分别为16333和16148Da，两者核苷酸序列和推导的氨基酸序列同源性分别为90．4％与81．2％。将推导的美国红鱼和大黄鱼β-珠蛋白氨基酸序列与其他鱼类及人β-珠蛋白氨基酸序列进行同源性比较，其同源性在35.5％～70．3％，它们与同属真口鱼纲的真鲷、黄尾鲱鱼、鲤鱼、虹鳟等的同源性较高，而与肉鳍鱼纲的肺鱼及板鳃纲的鲨鱼的同源性较低；二级结构预测表明，美国红鱼和大黄鱼β-珠蛋白存在类似哺乳动物的8个α-螺旋区，在近端与远端与血红素结合的组氨酸高度保守。
The β-globin gene and amino acid sequence of large yellow croaker （ Pseudosciaena crocea ） and red drum（ Sciaenops ocellatus ）were first described. The entire open reading frames of two β-gjobin genes were beth 447 bp long and codified 148 amino acid residues, The homology between them was 90.4 % for nucleotides and 81.2 % for deduced amino acids.Amino acid identity of two β-globin genes compared with those reported in other vertebrates including shark, teleosts and human, ranged from 35.5 to 70,3 %, The proximal histidine residues at the heme binding region and the important functional residues that were involved in the contacts with the heme and between interfaces were conserved.
出处
《安徽农业科学》
CAS
北大核心
2007年第9期2555-2557,共3页
Journal of Anhui Agricultural Sciences
基金
中国科学院海外杰出学者基金项目(2005-1-4
1-7)
国家973项目(2004CB117502)
国家自然科学基金项目(30671517
30528006)
