摘要
研究了曙红Y(EOSY)与牛血清白蛋白(BSA)作用的荧光光谱和紫外-可见吸收光谱特征,实验证明EOSY具有较强地猝灭BSA荧光强度的能力,分别用Stern-Volmer方程、Lineweaver-Burk方程和热力学方程等处理试验数据,得到了25℃时反应的结合常数为3.601×105L.mol-1,反应的ΔHθ,ΔGθ和ΔSθ分别为-20.66,-31.70 kJ.mol-1和37.06 J.K-1,为研究在人体生理条件下EOSY与BSA的结合方式、EOSY对BSA构象影响和细胞染色机理等提供了重要信息。
The binding of eosin Y to bovine serum albumin （BSA） was studied by fluorescence spectroscopy and ultraviolet spectrum. It was shown that this compound has a quite strong ability to quench the fluorescence from BSA. After analyzing the fluorescence quenching data according to Sterm-Volmer equation, Lineweaver-Burk equation and thermodynamic equation at 298. 15 K, the binding constant （KLB = 3. 601 ×10^5L · mol^-1 ） and thermodynamic parameters （△H^θ, ： - 22.66 kJ ~ mol 1, △G^θ： -31.30 kJ · mol 1 and △S^θ： 36. 32 J · K^-1）were obtained, which provide important information for researching the configuration modification of BSA caused by added eosin Y, biological effects of eosin Y in a living body, and dyeing mechanism of cells.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2006年第12期2306-2310,共5页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(30170010)
湖北省自然科学基金项目(2005ABA067)资助
关键词
曙红Y
牛血清白蛋白
荧光猝灭光谱
紫外吸收光谱
三维荧光光谱
热力学参数
Eosin Y
Bovine serum albumin
Fluorescence spectrum
Ultra-violet absorbance spectrum
Three dimensional fluorescence spectrum
Thermodynamic parameters
