摘要
目的:制备10-羟基喜树碱的半固体脂质纳米粒(HCPT-SSLN)并考察其稳定性。方法:采用高温乳化蒸发-低温固体法制备了HCPT-SSLN;用透射电镜考察了纳米粒的形态;用激光粒度仪测定了粒径和ξ电位;考察了其混悬液和冻干粉的物理稳定性。结果:HCPT-SSLN纳米粒平均粒径为130.5 nm,载药量为2.51%,包封率为79.19%,ξ电位为-33.1mV;室温(25℃)和4℃下放置6个月,纳米粒外观、粒径及包封率无明显变化,冻干粉比混悬液的物理稳定性更高。结论:本实验制备的HCPT-SSLN包封率和载药量较高,粒径分布均匀,稳定性良好。初步表明HCPT适合进行SSLN包裹。
Objective： To prepare 10-hydroxycamptothecin-semisolid lipid nanoparticles （HCPT-SSLN） and investigate its stability. Methods： HCPT-SSLN was prepared by the method of “emulsion evaporation at a high temperature and solidification at a low temperature” ; The morphology was examined by transmission electron microscope; The particle size and ξ potential were determined by laser granularity equipment; The physical stability of both suspl and freeze drying powder of HCPT-SSLN were investigated. Results： The mean particle size of the prepared HCPT-SSLN was 130. 5 nm, drug loading was 2.51% , entrapment efficiency was 79. 19%, ξ potential was -33, 1mV; Placed at room temperature and 4℃ for 6 months, the appearance, particle size and entrapment efficiency of HCPT-SSLN were all stable. Moreover, the freeze drying powder was more stable than the suspl. Conclusion： The HCPT-SSLN has high entrapment efficiency and drug loading, uniform particle size, good stability, which initially indicates that HCPT is fit for being incorporated into SSLN.
出处
《中药材》
CAS
CSCD
北大核心
2006年第1期63-66,共4页
Journal of Chinese Medicinal Materials
基金
广东省科技计划项目(2003A1070103)
