摘要
目的:制备羟基喜树碱的半固体脂质纳米粒(HCPT-SSLN),初步考察其体外释药规律。方法:采用乳化蒸发-低温固化法制备HCPT-SSLN;用激光粒度仪测定其粒径和ξ电位;考察其混悬液和冻干粉的物理稳定性;用透析法考察其体外释药性质。结果:HCPT-SSLN纳米粒平均粒径为130.5nm,载药量为2.51%,包封率为79.19%,ξ电位为-33.1mV;室温(25℃)和4℃下放置6个月,纳米粒冻干粉和混悬液外观、粒径及包封率无明显变化;体外释药规律符合Weibull方程lnln[1/(1-Q)]=0.263 3lnt+0.050 9(R2=0.948 5)。结论:制备的HCPT-SSLN包封率高,稳定性好,大小均匀,体外释药具有缓释特点。
OBJECTIVE To prepare hydroxycamptothecinsemisolid lipid nanoparticles （HCPT-SSLN） and study the release profile in vitro. METHODS HCPT-SSLN was prepared by the method of emulsion evaporation and solidification at a low temperature; the particle size and ξ potential were determined by laser granularity equipment;the physical stability of both suspension and lyophiled powder of HCPT-SSLN were investigated; dialyzer was used to study the release of HCPT in vitro. RESULTS The mean particle size of the prepared HCPT-SSLN was 130. 5 nm,drug loading（DL） was 2. 51%,entrapment efficiency（EE） was 79. 19% ,ξ potential was - 33. 1 mV; Placed at room temperature and 4℃ for 6 months,the appearance,particle size and entrapment efficiency of HCPT-SSLN were all stable; The release rule in vitro was subject to Weibull equation., lnln [-1/（1-Q）]=0.263 3lnt ＋ 0.050 9（R^2 = 0.948 5）. CONCLUSION The HCPT-SSLN has high entrapment efficiency,good stability, uniform particle size, and can retard drug release in vitro.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2007年第2期139-142,共4页
Chinese Journal of Hospital Pharmacy
基金
2003年广东省科技计划项目(编号:2003A1070103)
关键词
羟基喜树碱
半固体脂质纳米粒
体外释药
hydroxycamptothecin
semisolid lipid nanoparticles （SSLN）
drug-release in vitro
