摘要
背景:近期研究表明羟基喜树碱在一些器官移植过程中具有免疫抑制作用,但在角膜移植中的免疫抑制作用却未见报道。目的:观察羟基喜树碱对大鼠穿透性角膜移植术后免疫排斥反应的影响,并与经典抗排斥药环孢素A进行比较。方法:18只成年雌性Wistar大鼠为供体,36只成年雄性SD大鼠为受体,建立大鼠穿透性角膜移植动物模型。受体大鼠随机分为3组,羟基喜树碱组、环孢素A组和对照组,每组12只。羟基喜树碱组和环孢素A组分别在术后腹腔注射羟基喜树碱2.0mg/(kg·d)和环孢素A10mg/(kg·d),连续用药12d。对角膜植片进行临床观察,以混浊、水肿和新生血管3项指标作为临床评估标准,记录角膜植片存活时间。结果与结论:羟基喜树碱组、环孢素A组和对照组角膜植片的存活时间分别为(21±2),(19±2)和(11±2)d,各用药组与对照组比较角膜植片存活时间显著延长(P<0.01),羟基喜树碱组比环孢素A组植片存活时间明显延长(P<0.05)。实验结果提示羟基喜树碱能对大鼠穿透性角膜移植术后免疫排斥反应具有明显的抑制作用,可延长植片存活时间,抑制新生血管,其作用优于环孢素A。
BACKGROUND： Recent studies have shown that the hydroxycamptothecin has immunosuppression effect in some organ transplantations, but which in corneal transplantation has never been reported. OBJECTIVE： To observe the immunosuppresive effect by used hydrocamptothecin on allogeneic corneal transplantation in rat models and to compared with cyclosporin A. METHODS： Penetrating corneal transplantation model was established using 18 adult female Wistar rats as donors and 36 adult male Sprague Dawley rats as recipients. Recipients were randomly assigned to the three groups, hydroxycamptothecin group, cyclosporine A group and control group （n = 12）. Hydroxycamptothecin and cyclosporine A groups were respectively given with intraperitoneal injection of hydroxycamptothecin 2.0mg/kg per day and cyclopsorine A 10 mg/kg per day, starting on the first day after operation and for 12 consecutive days. Cornea transplant was observed, and turbid, edema and new vessels were considered as clinical assessment criteria. The corneal survival time was recorded. RESULTS AND CONCLUSION： The survival time of corneal graft in the hydroxycamptothecin group, cyclopsorin A group and control group respectively were （21±2）, （19±2） and （11±2） days. Graft survival time in each of treatment groups was significantly prolonged compared with control group （P 0.01）. Hydroxycamptothecin group was significantly superior compared with cyclosporine A group （P 0.05）. Experiment results indicated hydroxycamptothecin has a inhibitory effect on immunological rejection, a promotive effect on corneal allografts survival and a significantly preventive effect on vascular growth of corneal allograts, which would be superior to cyclopsorin A in rats following penetrating corneal transplantation.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第31期5778-5781,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
