摘要
普罗帕酮(propafenone,PPF)是一个高效低毒的新型抗心律失常药物(结构式见图1)。临床常用于防治室性或室上性快速型心律失常;起效迅速,疗效确切,深受临床的欢迎。由于PPF半衰期波动大,病人血药浓度差异很大;进一步研究发现PPF的主要活性代谢产物5-羟基普罗帕酮(5-hydroxypropafenone,5-OHP)及N-去丙基普罗帕酮(N-depropylpropafenone,NDP)(结构式见图1)在人体内均有较强的抗心律失常活性。
A rapid, sensitive and simple high performance liquid chromatographicmethod for the simultaneous determination of propafenone (PPF) and its metabolites(5-hydroxypropafenone, 5-OHP; N-depropylpropafenone, NDP) in serum hasbeen developed. Separation of PPF, 5-OHP and NDP was achieved by reversed phasechromatography using a mobile phase consisting of 57% methanol and 43% 10 mmol/Lpotassium dibasic phosphate (pH 2.7)at a flow rate of 1.0 mi/rain on a 5μm ODS-C18column. The eluent was monitored at 254 nm. The method showed a good linearity. The recoveries of PPF, 5-OHP and NDP werefound to be 99.54±2. 13%, 100.02±3.66% and 100.48±3.10%, respectively. Precisionstudies for both within day and day-to-day at different concentrations provided RSDvalues of less than 5%. Some commonly used drugs can be determined in the same procedurewithout interference except phenytoin. This method is well adapted to the therapeuticmonitoring of PPF treated patients, as well as for pharmacokinetic studies.
出处
《药学学报》
CAS
CSCD
北大核心
1992年第7期552-555,共4页
Acta Pharmaceutica Sinica
