摘要
目的 :建立血浆中普罗帕酮对映体浓度的立体选择性高效液相色谱测定法。 方法 :血浆标本经碱化以正己烷 /异戊醇 ( 99∶ 1)提取、吹干后与 GITC柱前衍生化 ,形成非对映体。以乙腈∶水∶冰醋酸 ( 60∶ 4 0∶ 0 .0 1)为流动相 ,经 C18ODS柱分离后在紫外 2 0 8nm处检测。 结果 :对映体曲线范围 2 5～ 80 0μg/ L ;最低检出限 <2 0μg/ L ;批内、批间平均 RSD均 <6% ;平均回收率 :10 2 .16% ;临床大多数常用药物对本法无干扰。 结论 :本法为普罗帕酮临床药动 -药效学研究提供了一种简便。
Objectives:A stereoselectie HPLC method was established to determine propafenone erantiomers in human plasma. Methods:Plasma sample was extracted with hexane iso- pentanol(99∶ 1 ) after basification.A precolumn derivation was performed by us- ing GITC.The enantiomers was eluted with acetonitrile- water- acetic acid(6 0∶ 40∶ 0 .0 1 ) on C1 8ODS column and detected under2 0 8nm. Results:The range of standard curve of each enantiomer was 2 5～ 80 0μg/ L . The lowest limit of detection was 2 0μg/ L or less. The average RSD of within or between day were all less than6 % and the average recovery rate was1 0 2 .1 6 % .Most commonly co- administered drug did not interfere the assay. Conclu- sions:The present study provided a simple and reliable quantitative method for the pharma- cokinetic and pharmacodynamic studies of propafenone enantiomers.
出处
《医学研究生学报》
CAS
1997年第4期315-318,共4页
Journal of Medical Postgraduates
