摘要
目的 建立粮食样品中玉米赤霉烯酮及其代谢物的毛细管电泳测定方法。方法 样品经液 -液超声波提取 ,C1 8小柱净化后 ,胶束电动毛细管电泳 (MEKC)测定。结果 玉米赤霉烯酮 (Zeralenone,ZON)、α- Zearalenol(α- ZOL)、β- Zearalenol(β- ZOL)、黄曲霉 B1 (Aflatoxin B1,AFT B1 )、赭曲霉 A(Ochratoxin A,Och A)的检出限分别为 :0 .0 0 84、0 .0 81、0 .14、0 .0 0 16、0 .0 31μg/m l。样品加标回收率 77.9%～ 10 3.1% ,相对标准偏差 0 .6 3%～ 1.98%。结论 该法灵敏、准确 。
Objective To establish a method for the determination of zearalenone and its metabolites in grains by capillary electrophoresis. Methods The samples were extracted with liquid liquid partition and ultrasonic extraction, cleaned up with C 18 cartridges, then determined by micellar electrokinetic capillary chromatography(MEKC). Results The limits of detection for Zearalenone(ZON),α Zearalenol(α ZOL),β Zearalenol(β ZOL),Aflatoxin B 1(AFT B 1) and Ochratoxin A(Och A)were 0.0084, 0.081, 0.14, 0.0016 and 0 031 μg/ml, respectively. Recoveries of all compounds were in the range of 77.9% 103.1%. Relative standard deviations (RSDs) were 0.63% 1.98%. Conclusion This sensitive and accurate method can be used in the determination of zearalenone and its metabolites in grains.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2003年第2期333-336,共4页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金重大课题 (批准号 3 0 0 3 0 12 03 0 0 70 678)资助
