摘要
目的:基于生物信息学确定肺腺癌(lung adenocarcinoma,LUAD)中与铁死亡和氧化应激相关的差异表达关键基因,并探讨其在免疫浸润及生存预后中的潜在作用。方法:通过分析基因表达综合(Gene Expression Omnibus,GEO)和癌症基因组图谱(the Cancer Genome Atlas,TCGA)数据库中的转录组数据,使用差异表达分析及与铁死亡和氧化应激相关基因交叉鉴定出关键基因。随后,采用生物信息学方法对关键基因进行生存分析和免疫细胞浸润分析,同时评估相关药物的互作,并利用单细胞RNA测序数据探讨关键基因在不同细胞类型中的表达特征。最后,对在湖州市第一人民医院收集的全血标本进行qPCR实验。结果:本研究识别出DUOX1和IL33两个基因,其在LUAD患者组织中的表达显著低于正常组织,并与患者生存期显著相关。免疫分析显示DUOX1和IL33与多种免疫细胞浸润之间存在显著相关性。单细胞分析进一步揭示了这两个基因在不同免疫细胞亚群中的表达模式,增强了对其在肿瘤微环境中作用的理解。此外,药物互作分析结果表明IL33可能成为LUAD的潜在治疗靶点,而qPCR实验结果与TCGA数据库验证结果一致。结论:DUOX1和IL33在肺腺癌中可能发挥重要作用,作为生物标志物的潜力为LUAD的早期诊断和靶向治疗提供了新的方向,为进一步研究提供了基础。
Objective:To identify differentially expressed key genes associated with iron death and oxidative stress in lung adenocarcinoma(LUAD) based on bioinformatics,and to explore their potential roles in immune infiltration and survival prognosis.Methods:By analyzing transcriptome data from Gene Expression Omnibus(GEO) and the Cancer Genome Atlas(TCGA) databases,differential expression analysis and cross identification with iron death and oxidative stress-related genes were used to identify key genes.Subsequently,bioinformatics methods were used to perform survival analysis and immune cell infiltration analysis on key genes,while evaluating drug interactions.Single cell RNA sequencing data were used to explore the expression characteristics of key genes in different cell types.Finally,qPCR experiments were conducted on the whole blood samples collected at the First People's Hospital of Huzhou.Results:This study identified two genes,DUOX1 and IL33,whose expression in LUAD tissue was significantly lower than that in normal tissue and significantly correlated with patient survival.Immunoassay showed a significant correlation between DUOX1 and IL33 and infiltration of multiple immune cells.Single cell analysis further revealed the expression patterns of these two genes in different immune cell subpopulations,enhancing our understanding of their roles in the tumor microenvironment.In addition,the drug interaction analysis results indicated that IL33 may become a potential therapeutic target for LUAD,and the qPCR experimental results were consistent with the validation results of the TCGA database.Conclusion:DUOX1 and IL33 may play important roles in LUAD,and their potential as biomarkers provides new directions for early diagnosis and targeted therapy of LUAD,laying the foundation for further research.
作者
李勇
施丹飞
李新民
嵇龙飞
LI Yong;SHI Danfei;LI Xinmin;JI Longfei(School of Medical Technology and Information Engineering,Zhejiang Chinese Medical University,Zhejiang Hangzhou 310053,China;The First Affiliated Hospital of Huzhou University,Zhejiang Huzhou 313000,China;Chongqing Hospital of Traditional Chinese Medicine,Chongqing 400000,China)
出处
《现代肿瘤医学》
2025年第3期409-416,共8页
Journal of Modern Oncology
基金
国家自然科学基金(编号:82202649)
浙江省医药卫生科技计划项目(编号:2023KY318)。
关键词
肺腺癌(LUAD)
铁死亡
氧化应激
免疫浸润
单细胞测序
lung adenocarcinoma(LUAD)
iron death
oxidative stress
immune infiltration
single-cell sequencing
