摘要
多重单细胞蛋白组技术已成为生物医学研究的热点,其中质谱流式成像(imaging mass cytometry,IMC)彻底解决了荧光基团间严重的串色问题,并弥补了单细胞测序技术缺失的组织空间信息。该技术能够在单张组织切片上同时标记数十种靶标,并在单细胞层面获得它们的表达水平及细胞定位,进行深度的细胞表型原位分析并从时间和空间水平直观描绘出单细胞蛋白组图谱,因其独特的技术优势逐渐成为肿瘤研究领域新兴的强有力工具。本文将详细介绍IMC的技术原理,并通过分析近期应用案例阐述其在细胞表型鉴定、生物标志物检测、肿瘤免疫调节判定、肿瘤异质性区分、临床预后指导及反应预测等肿瘤研究方面的应用进展,推动肿瘤空间组学与现有技术的进一步结合。
Multiplex single-cell proteomics technology has become a hot topic in biomedical research,among which imaging mass cytometry(IMC)completely solves the serious problem of cross-color between fluorophores and makes up for the lack of tissue spatial information in single-cell sequencing technology.This technique can label dozens of targets simultaneously on a single tissue section,obtain their expression levels and cell localization at the single-cell level,perform in-depth cell phenotypic in situ analysis,and visually depict single-cell proteome maps from the temporal and spatial levels,and due to its unique technical advantages,it has become a powerful tool in the field of tumor research.This article elaborates on the technical principle of IMC and summarizes the advances in the application of IMC in cell phenotype identification,biomarker detection,tumor immunomodulatory determination,tumor heterogeneity differentiation,clinical prognosis guidance,and response prediction by analyzing recent cases,so as to promote the further integration of tumor spatial omics with existing technologies.
作者
黄一薇
向廷秀
刘星河
冉静
赵毅
Huang Yiwei;Xiang Tingxiu;Liu Xinghe;Ran Jing;Zhao Yi(Oncology Laboratory,Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment,Chongqing University Cancer Hospital;School of Medicine,Chongqing University,Chongqing University Cancer Hospital)
出处
《重庆医科大学学报》
北大核心
2025年第1期6-13,共8页
Journal of Chongqing Medical University
基金
国家自然科学基金资助项目(编号:82172619)
重庆市科卫联合医学科研资助项目(编号:2024MSXM136)
重庆市沙坪坝区决策咨询与管理创新资助项目(编号:2023122)。
关键词
质谱流式成像
空间蛋白组
单细胞分析
肿瘤微环境
肿瘤标志物
imaging mass cytometry
spatial proteome
single-cell analysis
tumor microenvironment
tumor markers
