摘要
目的探讨人类白细胞抗原(human leukocyte antigen,HLA)基因多态性与乙型肝炎病毒(HBV)感染的相关性。方法收集云南省昆明市延安医院健康体检者静脉血样本501例,采用酶联免疫吸附试验(ELISA)检测HBV二对半,根据HBV二对半检测结果分为HBV携带组和既往感染组以及健康对照组3组,用序列特异性引物聚合酶链反应(polymerase chain reaction with sequence specific primers,PCR-SSP)基因分型技术检测HLA-A抗原的基因型,将HBV携带组和健康对照组以及HBV既往感染组和健康对照组的HLA-A基因多态性的分布频率进行比较。采用SPSS17.0软件进行数据统计分析。结果健康对照组HLA-A2阳性数占比47.49%,等位基因频率数占比31.29%;健康对照组基因分布频率总体与中华骨髓库发布的中国常见及确认的HLA-A等位基因表一致。HBV携带组HLA-A2阳性数占比63.04%,等位基因频率数占比42.23%,携带者的HLA-A2阳性率和等位基因频率差异有统计学意义(P<0.05);HBV既往感染组HLA-A2阳性数占比56.14%,等位基因频率数占比35.97%,既往感染组的HLA-A2阳性率和等位基因频率差异无统计学意义(P>0.05)。结论HLA-A2基因可能是慢性乙型肝炎HBV携带者的易感基因。
Objective To investigate the correlation between human leukocyte antigen(HLA)gene polymorphism and hepatitis B virus(HBV)infection.Methods Venous blood samples were collected from 501 healthy individuals undergoing physical examinations at Yan’an Hospital in Kunming,Yunnan Province.Enzyme linked immunosorbent assay(ELISA)was used to detect HBV halves.Based on the results of HBV half detection,the patients were divided into three groups:HBV carrier group,previous infection group,and healthy control group.The HLA-A antigen genotype was detected using polymerase chain reaction with sequence specific primers(PCR-SSP)genotyping technology,and the distribution frequency of HLA-A gene polymorphism was compared between HBV carrier group and healthy control group,as well as between previous infection group and healthy control group.SPSS17.0 software was used for data statistical analysis.Results In the healthy control group,the HLA-A2 positivity rate was 47.49%,and the allele frequency was 31.29%.The overall frequency of gene distribution in the healthy control group was consistent with the HLA-A allele table commonly and confirmed in China published by the Chinese Bone Marrow Bank.The HLA-A2 positivity rate and allele frequency in the HBV carrier group were 63.04%and 42.23%,respectively;The difference in HLA-A2 positivity rate and allele frequency among carriers was statistically significant(P<0.05).the HLA-A2 positivity rate and allele frequency in the HBV previous infection group were 56.14%and 35.97%,respectively,which did not significantly differ from those in the healthy control group(P>0.05).Conclusion HLA-A2 gene may be a susceptibility gene for chronic hepatitis B HBV carriers.
作者
帅莉
杨帅
叶峻杰
杨景成
付晓野
许彬
刘红伟
周律
康细林
高静
SHUAI Li;YANG Shuai;YE Junjie;YANG Jingcheng;FU Xiaoye;XU Bin;LIU Hongwei;Zhou Lü;KANG Xilin;GAO Jing(Yan’an Hospital Affiliated to Kunming Medical University,Kunming 650000;Yunnan Agricultural Vocational and Technical College,Kunming 650031;Yunnan Institute of Population and Family Planning Science and Technology,Kunming 650021;Yunnan Open University,Kunming 650504;Kunming Children’s Hospital,Kunming 650100,China)
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2024年第6期1037-1040,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
云南省高层次卫生健康技术人才培养计划资助(No.H-2018028)
昆明巿卫生健康委员会卫生科研项目(No.2023-11-01-003)。
