摘要
目的:探讨HLA-A*02等位基因及其亚型在云南宣威肺癌患者中的分布频率,为肺癌高发区的防治研究提供群体遗传学资料。方法:选取宣威籍肺癌患者与健康对照组,运用序列特异性引物基因分型技术(PCR-SSP)进行HLA-A位点及其HLA-A*02亚型分型。按照病例-对照研究方法分析HLA-A等位基因在两组之间频率分布的差异及肺癌HLA-A*02亚型多态性分布。结果:肺癌组与健康对照组HLA-A*02阳性率分别为68.75%及47.50%,肺癌组HLA-A*02阳性率显著增加(P=0.004,OR=2.432,95%CI=1.314～4.500);两组间HLA-A*02等位基因频率分别为41.67%及25.00%,具有显著性差异(P<0.05);宣威健康人群HLA-A等位基因分布频率总体特征与中国南方汉族人群一致;HLA-A*02阳性的肺癌组样本经高分辨检出HLA-A*0201、0203、0206、0207四种等位基因亚型,频率分别为29.73%、16.22%、13.51%、40.54%,符合中国南方汉族人群以A*0207为主的特征。结论:HLA-A*02可能与宣威肺癌易感相关,肺癌患者HLA-A*02亚型以A*0207为主,宣威地区人群属于南方汉族人群。
Objective：To study the frequency and distribution of HLA-A alleles and HLA-A＊02 subtypes in the lung cancer patients in Yunnan Xuanwei area.This study aims at providing the significant genetic information for lung cancer research and treatment.Methods：Genomic DNA samples were collected from lung cancer patients and healthy donors residing in Yunnan Xuanwei area.HLA-A alleles and HlA-A＊02 subtypes were typed by PCR-SSP assay.Case-control study was used to study the difference of frequency distribution between patients and normal controls.The polymorphisms of HLA-A＊02 were also observed in cancer patients.Results：The positive rates of HLA-A＊02 was significantly higher in cancer patients than in healthy controls （P=0.004,OR=2.432,95% CI=1.314-4.500）,which were 68.75% and 47.50%,respectively.The gene frequency of HLA-A＊02 was significantly higher in cancer patients than in healthy controls（P0.05）,which were 41.67% and 25.00%,respectively.The distribution character of HLA-A allele in Xuanwei healthy donors was similar to that in Han population in south China;Four different HLA-A＊02 subtypes （A＊0201,0203,0206 and 0207）were identified in HLA-A2 cancer patients,of which A＊0207 was the predominant allele as also found in Han population in south China.Conclusion：HLA-A＊02 may be associated with increased susceptibility to lung carcinoma in Xuanwei area.HLA-A＊0207 is the predominant allele in Xuanwei cancer patients.Xuanwei people may belong to the Han population in south China.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2010年第7期624-626,638,共4页
Chinese Journal of Immunology
基金
国家自然科学基金项目资助(No.30260103)
