摘要
目的对比ECLIA(ECLIA)与ELISA(ELISA)用于临床乙肝病毒血清学检验中的价值。方法选择2021年1月—2022年12月本院治疗的乙肝患者100例作为研究对象,分别通过ECLIA、ELISA检验进行乙肝病毒血清学检验,记录两种检验方式乙肝五项指标检测结果,对比组间统计学差异。计算两种检验方式检测乙肝五项指标阳性率。结果ECLIA与ELISA检验乙肝五项指标中HbsAb、HbcAb值组间比较差异无统计学意义(P>0.05),ECLIA检测乙肝五项指标中HbeAg、HbsAg及HbeAb结果均高于酶联免疫吸附法(P<0.05)。计算结果显示,ECLIA检测乙肝五项指标中HbeAg、HbsAg及HbeAb阳性率明显高于ELISA(P<0.05)。结论临床可通过ECLIA与ELISA方法进行乙肝病毒血清学检验工作,具有较高的准确性,二者相比,ECLIA准确率更高,能提供更准确的数据参考,值得运用推广。
Objective To compare value of chemiluminescence assay(ECLIA)and enzyme-linked immunosorbent assay(ELISA)in clinical serological detection of hepatitis B virus.Methods The paper chose 100 patients with hepatitis B in our hospital from January 2021 to December 2022 as research subjects,treated with hepatitis B virus serological tests by chemiluminescence method and enzyme-linked immunosorbent assay.Result of five hepatitis B indicators detected by two test methods were recorded.Statistical difference between control groups was compared.Positive rate of five indicators of hepatitis B detected by two test methods was calculated.Results There was no significant difference in HbsAb and HbcAb values of five hepatitis B indexes between chemiluminescence method and enzyme-linked immunosorbent assay(ELISA)(P>0.05).Levels of HbeAg,HbsAg and HbeAb in five hepatitis B indexes detected by chemiluminescence method were higher than those detected by enzyme-linked immunosorbent assay(p<0.05).Calculation showed,positive rate of HbeAg,HbsAg and HbeAb in five indicators of hepatitis B detected by chemiluminescence was significantly higher than ELISA(P<0.05).Conclusion Chemiluminescence method and enzyme-linked immunosorbent assay can be used in hepatitis B virus serological test in clinical practice,with high accuracy.Chemiluminescence method can achieve higher accuracy and provide more accurate data reference,which is worthy of promotion.
作者
令海英
LING Haiying(Laboratory Department,Wushan County Traditional Chinese Medicine Hospital,Tianshui,Gansu 741306)
出处
《智慧健康》
2023年第23期13-16,21,共5页
Smart Healthcare
关键词
ECLIA
ELISA
乙肝
病毒血清学检验
Chemiluminescence method
Enzyme linked immunosorbent assay
Hepatitis B
Viral serological testing
