摘要
目的:构建广泛耐药铜绿假单胞菌(XDR-PA)感染大鼠肺炎模型。方法:雄性SD大鼠24只随机分为空白组、低菌量组、中菌量组、高菌量组。空白组经气管滴注0.1毫升无菌生理盐水,低、中、高菌量组分别滴注0.1毫升的菌悬液(菌浓度依次为7.5×10^(9)、3×10^(10)、6×10^(10) CFU/mL)。造模后观察各组大鼠的精神状态、毛发、呼吸、活动、进食、体重等一般情况并绘制生存曲线;观察肺组织病理学特征及炎细胞浸润表现;通过肺组织匀浆细菌培养法对各组进行病原学鉴定。结果:空白组一般状态均正常,其他各组大鼠造模后出现精神萎靡,竖毛,呼吸急促,甚至口鼻出血,进食及活动减少,体重明显减轻,48 h内有不同程度的死亡,差异有统计学意义(P<0.05)。病理学结果显示,空白组大鼠肺泡结构完整,肺泡腔清晰无渗出;低、中菌量组肺组织均出现明显炎细胞浸润,肺泡结构破坏,肺泡间隔增厚,间质水肿,但中菌量组病理损伤更重,死亡率高达50%,低菌量组死亡率17%;高菌量组肺泡腔内可见红细胞、炎细胞及大量纤维素样渗出,有急性呼吸衰竭的病理学特征,死亡率高达67%。肺组织匀浆细菌培养结果显示空白组无菌落生长;低、中、高菌量组均可见PA菌落生长。结论:气管内滴注低菌量(0.1 mL 7.5×10^(9) CFU/mL)XDR-PA菌悬液,可成功构建XDR-PA感染大鼠肺炎模型。
Objective:To establish extensively drug-resistant Pseudomonas aeruginosa(XDR-PA)infection-induced pneu-monia model in rats.Methods:Twenty-four male SD rats were randomly divided into the blank group,the low bacterial group,the medium bacterial group,and the high bacterial group.The low,medium and high bacterial groups were given intratracheal in-stillation of 0.1 mL of bacterial suspension(bacterial concentration in turn is 7.5×10^(9)、3×10^(10)、6×10^(10)CFU/mL),while the blank group were given the same volume of sterile normal saline.After modeling,the general conditions of rats in each group were ob-served,including mental state,hair,respiration,activity,eating,weight,and the survival curve was drawn.The pathological characteristics of lung tissue and the infiltration of inflammatory cells were observed.Pathogenic identification of each group was carried out by bacterial culture of lung tissue homogenate.Results:The general state of the blank group was normal,and the rats in other groups showed signs of mental depression,bristling,shortness of breath,even oral and nasal bleeding,decreased food in-take and activity,and significant weight loss,and different degrees of death within 48 hours,the difference was statistically signifi-cant(P<0.05).Pathological results showed that the alveolar structure of rats in the blank group was complete,and the alveolar space was clear without exudation.The lung tissue of the low and medium bacterial groups showed obvious inflammatory cell infil-tration,alveolar structure destruction,alveolar septum thickening,interstitial edema,but the pathological damage of the medium group was more severe,with a mortality rate of up to 50%,and the mortality rate of the low bacterial group was 17%.In the high bacterial group,red blood cells,inflammatory cells and a large amount of fibrin-like exudation can be seen in the alveolar space,which has the pathological characteristics of acute respiratory failure,and the mortality rate is as high as 67%.The results of bacte-rial culture of lung tissue homogenate showed that the blank group had no bacterial colonies,while PA colony growth can be seen in low,medium and high bacterial groups.Conclusion:Intratracheal instillation of low bacterial count(0.1 mL of 7.5×10^(9) CFU/mL)XDR-PA bacterial suspension can successfully construct a rat pneumonia model of XDR-PA infection.
作者
安然
刘奉云
赵启亮
蔡文欣
孙宏源
陈明虎
常力
尹延重
刘凯
张慧琪
刘旻
AN Ran;LIU Feng-yun;ZHAO Qi-iang;CAI Wen-xin;SUN Hong-yuan;CHEN Ming-hu;CHANG Li;YIN Yan-zhong;LIU Kai;ZHANG Hui-qi;LIU Min(Department of Infectious Diseases,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 300193,China;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)
出处
《海南医学院学报》
2023年第15期1128-1134,共7页
Journal of Hainan Medical University
基金
天津市中医药重点领域科技项目(2021010)
天津中医药大学第一附属医院学科发展基金(XKJJ201734)。
关键词
广泛耐药铜绿假单胞菌
肺炎
动物模型
大鼠
Extensively drug-resistant Pseudomonas aeruginosa
Pneumonia
Animal model
Rat
