摘要
目的研究八角茴香复方对脂多糖(LPS)诱导的大鼠急性肺炎模型的抗炎作用。方法取48只大鼠随机分为6组,分别为对照组、模型组、阳性对照组及复方低、中、高剂量组,灌胃给药2 d,末次给药1 h后使用鼻腔滴入法制备急性肺炎模型,继续给药3 d后腹腔注射2%戊巴比妥钠麻醉。采用ELISA试剂盒检测各组大鼠支气管肺泡灌洗液(BALF)中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)含量的变化;血液分析仪检测血液白细胞种类和数量,并对BALF的炎性细胞进行分类和计数;通过中性粒细胞/淋巴粒细胞比值(NLR)评估肺炎预后价值;HE染色观察肺组织形态学变化。结果八角茴香复方对LPS诱导的大鼠急性肺损伤具有较好的抗炎作用,可降低大鼠BALF及血清中IL-1β、IL-6、TNF-α的表达,降低炎性细胞的种类和数量。结论八角茴香复方对LPS诱导的大鼠急性肺炎模型具有抗炎作用,其机制可能通过降低IL-1β、IL-6、TNF-α的表达来实现的。
Objective:To study theanti-inflammatory effect of Compound Star Aniseon lipopolysaccharide(LPS)induced acute pneumonia in rats.Methods:A total of 48 rats were randomly divided into 6 groups:nor-mal control group,model group,positive control group,and Compound Star Anise low-dose group,medium-dose group,high dose group.The drug was pre-administered for two days,and LPS was injected into the nasal 1 h after the last administration to prepare the acute pneumonia model.Then the animals were sacrificed by 2%sodium pen-tobarbital intraperitoneal after 3 days of dosing later.The level changes of inflammatory factors interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6)in the bronchoalveolar lavage fluid(BALF)of rats in each group were detected by ELISA kit;hematology analyzer was used to detect the type and number of white blood cells,and the inflammatory cells in BALF were classified and counted;evaluation of the prognostic val-ue of pneumonia was conducted by the neutrophil/lymphocyte ratio(NLR);HE staining was applied to observe the morphological changes of lung tissue.Results:The pre-administration of Compound Star Anise had a strong pro-tective effect on LPS-induced lung injury in rats,which reduced the inflammatory factors IL-1β,IL-6,and TNF-αin rats'BALF,reduced the type and number of inflammatory cells.Conclusion:The anti-inflammatory protec-tive effect of Compound Star Anise on LPS-induced acute pneumonia in rats may be related to the down-expres-sion of inflammatory factors IL-1β,IL-6,and TNF-α.
作者
张孟丽
吴芳芳
谭栀恩
林浩
李太平
何艳梅
欧敏
王喜军
Zhang Mengli;Wu Fangfang;Tan Zhien;Lin Hao;Li Taiping;He Yanmei;Ou Min;Wang Xjun(National Engineering Laboratory for the Development of Southwestern Endangered Medicinal Materials,Guangxi Botanical Garden of Medicinal Plant,Guangxi,Nanning 530023,China)
出处
《中国中医急症》
2023年第6期951-953,991,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
国家自然科学基金项目(82003932)
中央引导地方科技创新基地建设类项目(桂科ZY21195044)
广西重点研发计划(桂科AB20159029)
广西科技计划项目(AD18126013)。
关键词
急性肺炎
八角茴香复方
化学成分
炎性因子
大鼠
Acute pneumonia
Compound Star Anise
Chemical composition
Inflammatory factors
Rats
