摘要
【目的】了解2021年上海市B亚属人腺病毒分离株的基因特征。【方法】对1例腺病毒55型感染导致的急性结膜炎病例进行病原体分离鉴定及全基因组测序,分析主要抗原蛋白六邻体(Hexon)、五邻体(Penton)、纤维突蛋白(Fiber)基因及全基因组序列的进化特征。【结果】该病毒株属于B亚属B2亚组,Hexon基因与HAdV-11进化关系相近,而Fiber和Penton基因则与HAdV-14进化关系更为接近。核苷酸序列比对分析显示,该病毒与近期国内外流行的HAdV-55病毒株同源性均超过99.90%,与广东株73-GD_CHN_2016核苷酸相似度最高,在E1B、L4、E3和L5基因区存在氨基酸变异。【结论】上海株(MH2021001)为腺病毒B亚属55型,该病毒基因组未发生多基因片段重组,与国内流行病毒株相比存在部分氨基酸变异,其生物学特性有待进一步研究。
[Objective]To determine the genomic characteristics of a subgenus B human adenovirus strain isolated in Shanghai in 2021.[Methods]An adenovirus type 55 strain was isolated and identified from a patient with acute hemorrhagic conjunctivitis(AHC).Complete genome of the strain was obtained using the next-generation sequencing(NGS).Phylogenetic trees were reconstructed based on the sequences of Hexon,Fiber,Penton and complete genome to genomically characterize this strain.[Results]Phylogenetic analysis based on the complete genome classified this strain(MH2021001)into subgenus B,subspecies B2 of HAdV-55.Hexon gene of MH2021001 had close phylogenetic relationship with HAdV-11,while Fiber and Penton genes had close relationship with HAdV-14.The MH2021001 showed high nucleotide identity with currently prevalent HAdV⁃55 strains(>99.90%).The complete genome had 99.96%nucleotide identity to the 73-GD_CHN_2016 strain isolated in Guangdong.In addition,the amino acid sequence of MH2021001 had several substitutions in regions coding for E1B,L4,E3 and L5.[Conclusion]This strain has been classified to HAdV-B55.No recombination event is identified in the complete genome.Due to multiple amino acid substitutions,the biological characteristics of the strain need to be further identified.
作者
刘嘉婧
崔晓青
张万菊
房方皓
彭雅君
陈敏
张靖翊
滕峥
LIU Jiajing;CUI Xiaoqing;ZHANG Wanju;FANG Fanghao;PENG Yajun;CHEN Min;ZHANG Jingyi;TENG Zheng(Institute of Microbiology Laboratory,Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China;Institute of Microbiology Laboratory,Shanghai Institute of Preventive Medicine,Shanghai 200336,China;Shanghai Eye Disease Treatment and Prevention Center,Shanghai 200040,China)
出处
《上海预防医学》
CAS
2023年第4期332-337,共6页
Shanghai Journal of Preventive Medicine
基金
上海市疾病预防控制青年骨干人才培养项目(21QNGG03)
上海市公共卫生建设三年行动计划(GWV-2)。
关键词
人腺病毒55型
急性出血性结膜炎
全基因组测序
基因特征
human adenovirus-55
acute hemorrhagic conjunctivitis
complete genome sequencing
genomic characteristics
