摘要
该研究利用秀丽隐杆线虫(Caenorhabditis elegans)模型研究了羊乳酪蛋白酶解物(Goat Milk Casein Hydrolysates,GMCH)的抗衰老及体内外抗氧化作用。通过对比GMCH实验组和大肠杆菌对照组秀丽线虫的寿命、运动能力、脂褐素水平等指标来研究GMCH的抗衰老作用,通过自由基清除实验以及测定线虫体内抗氧化酶活性评价GMCH的体内外抗氧化活力,同时测定秀丽线虫在过氧化氢、百草枯及热应激条件下的寿命。结果表明,与对照组相比,喂食0.1mg/mL羊乳酪蛋白酶解物的线虫的平均寿命延长9.33%,体内脂褐素水平降低了65.00%,同时GMCH对线虫的生殖能力和运动能力没有负面影响,对线虫氧化应激和热应激有一定的保护作用;GMCH具有一定的体外抗氧化能力并且能够提高线虫体内抗氧化酶活力,与对照组相比,实验组SOD活力提高43.30%、CAT活力提高124.40%、GSH活力提高176.90%、MDA含量降低78.70%。综上所述,GMCH能够延长线虫的寿命,降低线虫体内脂褐素积累,提高线虫在应激条件下的抵抗能力和体内抗氧化酶活力,具有较好的抗衰老活性。
In this study,the anti-aging and antioxidant activities of goat milk casein hydrolysates(GMCH)were studied in vitro and in vivo using a Caenorhabditis elegans model.The anti-aging effect of GMCH was studied by comparing the lifespan,exercise ability,lipofuscin level and other indicators of Caenorhabditis elegans between the GMCH experimental group and the E.coli OP50 control group.The in vitro and in vivo antioxidant activities of GMCH were evaluated by the free radical scavenging experiment sand the activities of antioxidant enzymes in C.elegans.At the same time,the lifespan of C.elegans under hydrogen peroxide,paraquat and heat stress conditions were determined.The results showed that compared with the control group,the average lifespan of the nematode fed with 0.1 mg/mL goat casein hydrolysate was prolonged by 9.33%,and the lipofuscin level in the body decreased by 65.00%.Meanwhile,GMCH had no negative impact on the reproductive and motor ability of the nematode,and had a certain protective effect on oxidative stress and heat stress of the nematode.GMCH had a certain in vitro antioxidant capacity and could improve the activities of antioxidant enzymes in vivo.Compared with the control group,the SOD activity of the experimental group increased by 43.30%,the CAT activity increased by 124.40%,the GSH activity increased by 176.90%,and the MDA content decreased by 78.70%.In summary,GMCH can prolong the lifespan of nematodes,reduce lipofuscin accumulation in nematodes,improve the resistance and the activities of antioxidant enzyme inside the body of nematodes under stress conditions,thereby having a good anti-aging activity.
作者
朱秋轶
宋玉
刘星雨
刘果
潘婉婷
曹素芳
陈薛琪
乔子骄
彭小雨
潘丽娜
曹庸
ZHU Qiuyi;SONG Yu;LIU Xingyu;LIU Guo;PAN Wanting;CAO Sufang;CHEN Xueqi;QIAO Zijiao;PENG Xiaoyu;PAN Lina;CAO Yong(College of Food Science,South China Agricultural University,Guangzhou 510642,China;Australian Dairy(China)Co.Ltd.,Changsha 410000,China)
出处
《现代食品科技》
CAS
北大核心
2023年第6期1-9,共9页
Modern Food Science and Technology
基金
国家自然科学基金项目(31972078)
中国博士后面上项目(2020M672651)
湖南省营养健康品工程技术研究中心创新平台与人才计划(2019TP2066)。
