摘要
目的:从枸杞子中分离纯化得到枸杞子多糖,并对其体外抗氧化活性及抗衰老作用进行研究。方法:通过水提醇沉法提取得到枸杞子粗多糖,再经过Sevage试剂除蛋白、DEAE-52纤维素离子交换树脂从粗多糖中分离纯化得到枸杞子多糖LBP。通过测定LBP对DPPH自由基、羟基自由基和ABTS+自由基的清除能力及对Fe^(3+)还原能力,评价LBP的体外抗氧化能力,采用D-半乳糖诱导建立衰老小鼠模型,给药结束后,比较各组小鼠的体质量及脏器指数,检测血清、肝和脑组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPx)和过氧化氢酶(CAT)的水平,蛋白质印迹(Western blotting)法检测肝组织中核内因子-E2-相关因子(Nrf-2)和血红素氧合酶-1(HO-1)的蛋白表达。结果:分离纯化得到的多糖LBP含量为86.64%±2.34%;LBP对DPPH自由基、羟基自由基和ABTS^(+)自由基清除能力的IC_(50)值分别为0.2081±0.0182、0.7132±0.0220和0.3646±0.0138 mg/mL;与模型组相比,阳性组和LBP高剂量组显著提高小鼠体质量(P<0.05或P<0.01),脏器指数显著升高(P<0.05或P<0.01);阳性组和LBP高剂量组可显著提高小鼠血清、肝和脑组织中SOD、CAT和GSHPx水平(P<0.05或P<0.01),并极显著降低MDA水平(P<0.01),肝脏组织中Nrf-2蛋白表达水平除LBP低剂量外均显著升高(P<0.05),HO-1蛋白表达水平在各组中均显著升高(P<0.05或P<0.01)。结论:枸杞子多糖LBP具有较强的抗氧化能力和一定的抗衰老作用,其作用机制可能与Nrf-2/HO-1信号通路有关。
Objective:To isolate and purify the polysaccharides from Lycium barbarum and study its in vitro antioxidant activity and in vivo anti-aging effect.Methods:Crude polysaccharides from Lycium barbarum were obtained by water extraction and alcohol precipitation,and then LBP was isolated and purified from crude polysaccharides by Sevage reagent and DEAE-52 cellulose ion exchange resin.By measuring the scavenging ability of LBP to DPPH free radical,hydroxyl radical and ABTS+free radical,and Fe^(3+)reduction,the antioxidant ability of LBP in vitro was evaluated.The aging mouse model was established by D-galactose.After administration,the body weights and organ indexes of mice in each group were compared.The levels of MDA,SOD,GSH-Px and CAT in serum,liver and brain were measured.The protein expression of Nrf-2 and HO-1 in liver tissue was detected by Western blotting.Results:The content of LBP after isolation and purification was 86.64%±2.34%.The IC_(50) values of scavenging ability of LBP to DPPH free radical,hydroxyl radical and ABTS^(+)free radical were 0.2081±0.0182,0.7132±0.0220 and 0.3646±0.0138 mg/mL,respectively.Compared with the model group,the body weights and organ indexes of the mice in positive and high dose of LBP groups were significantly increased(P<0.05 or P<0.01).The levels of SOD,CAT and GSH-Px in serum,liver and brain of mice in positive and high dose of LBP groups were significantly increased,and the level of MDA was significantly decreased(P<0.01).The expression level of Nrf-2 protein in liver tissue increased significantly except for in the low dose of LBP group(P<0.05),and the expression level of HO-1 protein increased significantly in all groups(P<0.05 or P<0.01).Conclusion:LBP from Lycium barbarum has strong antioxidant ability and certain anti-aging effect,and its mechanism may be related to Nrf-2/HO-1 signal pathway.
作者
王聪颖
黄小强
徐玥玥
茹国华
WANG Congying;HUANG Xiaoqiang;XU Yueyue;RU Guohua(Medical School,Huanghe Science&Technology College,Zhengzhou 450063,China;College of Pharmacy,Henan University,Kaifeng 475004,China)
出处
《食品工业科技》
CAS
北大核心
2024年第19期1-8,共8页
Science and Technology of Food Industry
基金
河南省科技攻关项目(242102311266)
河南省医学教育研究项目(wjlx2022199)
河南省研究生教育改革与质量提升工程项目(YJS2023JD68)。
