摘要
目的探讨α-硫辛酸(ALA)对兔耳增生性瘢痕(HTS)形成的影响及其机制。方法选取18只新西兰大白兔,随机分为正常组、模型组、4%ALA组,用药28 d后采用苏木精-伊红(HE)染色、马松(Masson)染色及免疫组织化学染色评估瘢痕相关指标的变化;评估氧化应激相关指标的变化;采用蛋白免疫印迹法检测抗氧化通路相关蛋白的表达水平。结果4%ALA组较模型组瘢痕颜色变浅、质地变软、体积明显缩小。HE、Masson染色和免疫组织化学染色结果显示4%ALA组较模型组成纤维细胞数目减少,纤维化程度减轻。4%ALA组中,丙二醛(MDA)含量低于模型组(P<0.001),羟脯氨酸(Hyp)含量低于模型组(P<0.01),谷胱甘肽(GSH)水平较模型组升高(P<0.01),T-SOD活力较模型组升高(P<0.001)。蛋白免疫印迹结果显示4%ALA组NRF2含量较模型组增加,其下游抗氧化蛋白的表达水平升高。结论ALA上调NRF2信号通路以降低兔耳HTS内氧化应激水平从而抑制瘢痕增生。
Objective To investigate the effect and mechanism of α-lipoic acid(ALA) on the formation of hypertrophic scar(HTS) in rabbit ears. Methods Eighteen New Zealand white rabbits were randomly divided into normal group, model group and 4%ALA group. After 28 days of treatment, the changes of scar related indexes were evaluated by hematoxylin-eosin(HE) staining, Masson pine(Masson) staining and immunohistochemical staining, the changes of oxidative stress related indexes were evaluated, and the expression of antioxidant pathway related proteins was detected by Western blotting. Results The color, texture and volume of scar in 4%ALA group were lighter and softer than those in model group. The results of HE, Masson staining and immunohistochemical staining showed that the number of fibrous cells and the degree of fibrosis in the 4%ALA group were less than those in the model group. In the 4%ALA group, the content of malondialdehyde(MDA) was lower than that of the model group(P<0.001), the content of hydroxyproline(Hyp) was lower than that of the model group(P<0.01), the level of glutathione(GSH) was higher than that of the model group(P<0.01), the activity of T-SOD was higher than that of the model group(P<0.001). Western blotting showed that the content of NRF2 and the expression level of downstream antioxidant proteins in the 4%ALA group were higher than those in the model group. Conclusion ALA up-regulates NRF2 signal pathway to reduce the level of oxidative stress in HTS and inhibit scar proliferation in rabbit ears.
作者
倪子樵
张锦松
高翔
朱秋璇
王鑫
黄海年
朱飞
Ni Ziqiao;Zhang Jinsong;Gao Xiang;Zhu Qiuxuan;Wang Xin;Huang Hainian;Zhu Fei(Dept of Plastic Surgery,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;Dept of Plastic Surgery,North Branch of the First Affiliated Hospital of Anhui Medical University,Hefei 230012)
出处
《安徽医科大学学报》
CAS
北大核心
2022年第7期1060-1065,共6页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:30973124)
安徽医科大学校基金(编号:2019xkj058)。
