摘要
目的:建立重症联合免疫缺陷(SCID)小鼠增生性瘢痕模型,并观察辛伐他汀乳膏外用对瘢痕的抑制作用。方法:取4周龄SCID小鼠12只,将手术切除的人类瘢痕组织皮片移植到其背部,1周后将小鼠随机分为3组,分别为模型组、乳膏基质组和1%辛伐他汀乳膏组,连续外用药物2周后,取各组移植瘢痕组织行HE染色、Masson染色;碱水解-分光光度法检测瘢痕组织中羟脯氨酸(HPr)含量。结果:植皮后1周,鼠背可观察到黄褐色、隆起于皮面、质地柔韧的类瘢痕组织,组织病理学可见排列紊乱的粗大胶原束,呈结节状或漩涡状排列,符合增生性瘢痕的特点。外用1%辛伐他汀乳膏2周后,真皮纤维化程度较模型组有所缓解,胶原含量减少,差异具有统计学意义(P<0.01)。结论:SCID鼠增生性瘢痕模型类似于人类增生性瘢痕,1%辛伐他汀乳膏可抑制该动物模型瘢痕组织增生。
Objective To investigate the inhibitory effect of simvastatin cream on established hypertrophic scar model of severe combined immunodeficiency (SCID) mice.Methods Human split-thickness skin grafts of hypertrophic scar were transplanted onto the dorsum of 12 four weeks old SCID mice.After one week,the mice were randomly divided into 3 groups:model control group,vehicle only group and 1% simvastatin cream group.After two consecutive weeks of treatment,the hypertrophic scar xenografts were harvested and given biopsy for histology by HE and Masson staining.Besides,alkaline hydrolysis-spectrophotometry was used to determine the level of hydroxyproline in hypertrophic scar xenografts.Results After one week of transplantation,the xenografts showed characteristic human hypertrophic scar including a yellowish brown,firm and elevated appearance.Histopathology showed disordered thick collagen fibrils arranged in nodular or whirlpool shape.After two weeks treatment of 1% simvastatin cream,the dermal fibrosis was alleviated and collagen content was decreased compared with model control group,the difference was statistically significant(P<0.01).Conclusion The hypertrophic scar model of SCID mice is similar to that of human hypertrophic scar and 1% simvastatin cream could inhibit the scar development in this animal model.
作者
陈俊伟
李帆
杨艳清
CHEN Jun-wei;LI Fan;YANG Yan-qing(Department of Plastic Surgery,the Third Hospital of Wuhan,Tongren Hospital of Wuhan University,Wuhan 430060,Hubei,China)
出处
《中国美容医学》
CAS
2020年第1期55-58,共4页
Chinese Journal of Aesthetic Medicine
基金
武汉市整形美容临床医学研究中心项目{武科计[2016]42号,项目编号:17}
