摘要
目的观察血管内皮生长因子(VEGF)抗体靶向血管治疗对人增生性瘢痕Ⅰ型胶原蛋白在裸鼠体内表达的影响。方法将1％TBSA深Ⅱ度创面愈合后的增生性瘢痕组织块(取自1例女性烧伤患者)植入48只BALA／C裸鼠肩胛部皮下,建立裸鼠增生性瘢痕移植模型。术后3周,将裸鼠分为大剂量组、中剂量组、小剂量组及对照组,每组12只,分别用0．01 mol／L灭菌磷酸盐缓冲液(PBS)稀释的15、10、5μg／ml VEGF单克隆抗体200μl以及等量、同浓度的PBS进行瘢痕内直接注射,每周2次,持续3周。术后45 d,测量各组裸鼠瘢痕组织的大小,计算体积;以HE染色行组织学观察;采用逆转录聚合酶链反应与蛋白质印迹法分析瘢痕组织Ⅰ型前胶原蛋白mRNA和Ⅰ型胶原蛋白的表达。结果大剂量组、中剂量组、小剂量组瘢痕体积分别为(55．3±4．1)、(67．9±5．7)、(78．9±5．5)mm3;与对照组(85．0±7．3)mm3比较,大剂量组、中剂量组瘢痕体积明显变小(P< 0．05)。大剂量组、中剂量组血管和成纤维细胞较少,胶原纤维减少,排列较整齐。与对照组比较,大剂量组和中剂量组Ⅰ型前胶原蛋白mRNA和Ⅰ型胶原蛋白表达明显降低;小剂量组与之接近。结论VEGF抗体靶向血管治疗可抑制增生性瘢痕血管形成、胶原表达及瘢痕生长。
Objective To investigate the influence of the vascular endothelial growth factor（VEGF） antibody targeted vascular therapy on the expression of human collagen type I in hyperplastic scar of nude mice. Methods The hyperplastic scar from one femal burn patient with 1% TBSA deep-partial thickness burns were implanted into subcutaneous skin of scapular region of 48 nude mice. Three weeks later, the nude mice were divide into large dose （LA） , medium dose （MD）, small dose （SD） and control groups, with 12 mice in each group. The mice in LA,MD and SD groups were injected with 200 μl of 15,10, 5 μg/ml VEGF monoclonal antibody diluted in 0.01 mol/L PBS, respectively in the scar twice a week for 3 weeks, while those in C group were injected with equal amount of 0.01 mol/L PBS. The area and volume of the scar in each group were calculated and histological changes were observed, and the expression of collagen type I mRNA and its protein in each group were determined 3 days after treatment. Results The volume of scar in LA, MD, SD and C groups were （55.3 ±4.1, 67.9 ±5.7, 78.9±5.5, 85.0 ±7.3） mm^3, respectively. Compared with that in C group, the volume of the scar were significantly decreased in AD and MD groups （ P 〈0.05）. A few number of vessels and fibroblasts were observed in LD, MD groups, with decreased number of collagen fibers arranged in order. Compared with that in C group ,The expression of procollagen type I mRNA and its protein in C group was obviously higher than those in LD and MD groups （ P 〈 0.05 ） , but it was similar to those in SD group. Conclusion VEGF targeted vascular therapy is beneficial for the inhibition of the angiopoiesis of hyperplastic scar, the expression of collagen , and the growth of scar.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2006年第6期427-430,共4页
Chinese Journal of Burns
基金
广西科学基金资助项目(0339078)
关键词
血管内皮生长因子类
胶原Ⅰ型
靶向血管治疗
增生性瘢痕
Vascular endothelial growth factors
Collagen type Ⅰ
Endothelial cell-targeted therapy
Hypertrophic scar
