摘要
[目的]对种植于天津滨海的自根苗转AhDREB1基因毛白杨((Populus tomentosa×P.bolleana)×P.tomentosa)以及种植于沧州盐山的嫁接苗转基因株系进行安全性研究,评估转基因毛白杨对环境可能造成的影响。[方法]利用PCR扩增以及电泳技术,对种植在天津滨海和沧州盐山实验田的4年生转基因毛白杨基因组DNA、土壤DNA、沧州嫁接苗砧木DNA以及抗性微生物基因组DNA进行PCR特异性检测。对天津实验田土壤中根际微生物数量进行连续动态监测(3月、4月、5月)。分别在天津滨海和沧州盐山实验田模拟自然条件下转基因毛白杨植株枯落物掉落,检测不同生长阶段的不同器官(新生嫩芽、多年生枝条、新生根尖、多年生侧根、新生嫩叶、当年生老叶)的降解时间。通过化感实验,检测天津滨海和沧州盐山实验田转基因毛白杨植株叶片对白菜(Brassica pekinensis)种子的生长是否造成影响。[结果]电泳结果显示:外源基因稳定存在于转基因毛白杨基因组中,实验田土壤DNA、抗性微生物DNA样品以及沧州嫁接苗砧木DNA中均未出现目的基因片段;天津非转基因植株和转基因植株的根际土壤中可培养微生物数量没有显著差异;转基因毛白杨的枯落物,无论是飘落在杂草表面、土壤表面还是埋入土壤,2个月后外源基因均被降解。化感实验结果显示:非转基因植株以及转基因植株对白菜种子的胚轴和胚根生长的影响没有显著差异。[结论]未发现实验田种植4 a的转基因毛白杨对周围的环境造成影响。
[Objective]To study the safety of transgenic Populus tomentosa with AhDREB1 gene from selfrooted seedlings planted in Binhai of Tianjin and transgenic seedlings planted in Yanshan of Hebei in order to evaluate the possible impact of transgenic Populus tomentosa on the environment.[Method]PCR amplification and electrophoresis were used to detect the genomic DNA of the 4-year-old transgenic P.tomentosa,soil DNA,root DNA of grafted seedlings from Yanshan and the genomic DNA of resistant microorganisms in Binhai.The amount of rhizosphere microorganisms in the soil of Binhai trial plot was monitored continuously(March,April and May).The degradation time of different organs(new shoots,perennial branches,new root tips,perennial lateral roots,new young leaves and old leaves)at different growth stages of transgenic P.tomentosa plants were measured in Binhai and Yanshan trial plots under simulated natural conditions.The allelopathic test was conducted to determine whether the transgenic leaves of P.tomentosa had any effect on the growth of Brassica pekinensis seeds.[Result]The electrophoretic results showed that exogenous genes stably existed in the genome of transgenic P.tomentosa,and no target gene fragment was found in soil DNA,root stock DNA of Yanshan grafted seedlings and DNA samples of resistant microorganisms.There was no significant difference in the amount of culturable microorganisms in the rhizosphere soil between non-transgenic plants and transgenic plants in Binhai.Exogenous genes were degraded in the litter of transgenic P.tomentosa after 2 months,whether it fell on the weed surface,soil surface or buried in soil.The allelopathic results showed that there was no significant difference between the non-transgenic plants and the transgenic plants on the growth of the hypocotyl and radicle of Brassica pekinensis seed.[Conclusion]No effect of transgenic P.tomentosa on the surrounding environment is found after 4 years of planting in the trial plot.
作者
侯荣轩
赵烨
田彦挺
佀化玙
张华新
李娟
纪清巨
杨庆山
孙宇涵
李云
HOU Rong-xuan;ZHAO Ye;TIAN Yan-ting;SI Hua-yu;ZHANG Hua-xin;LI Juan;JI Qing-ju;YANG Qing-shan;SUN Yu-han;LI Yun(National Engineering Laboratory for Tree Breeding,Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China;Chinese Academy of Forestry,Beijing 100091,China;Yanshan Natural Resources and Planning Bureau,Cangzhou 061300,Hebei,China;Seedling Station of Cangzhou Natural Resources and Planning Bureau,Cangzhou 061001,Hebei,China;Shandong Academy of Forestry,Ji'nan 250014,Shandong,China)
出处
《林业科学研究》
CSCD
北大核心
2022年第2期56-66,共11页
Forest Research
基金
国家科技重大专项(2018ZX08020002-003-002)
国家林业局科技发展中心项目(JC-2017-02)
国家自然科学基金青年基金项目(31800559)
国家“863”课题(2013AA102703)。
关键词
转基因毛白杨
嫁接苗与自根苗
转基因安全性评价
基因水平转移
土壤微生物数量
transgenic plant Populus tomentosa
grafted seedlings self-rooted seedlings
transgenic safety evaluation
horizontal gene transfer
soil microbial population
