摘要
玉米作为我国粮食储备的主要粮种之一,因其水分含量高、营养物质丰富、带菌量大等特点极易发生霉变、真菌毒素超标。本实验采用水热法优化制备了一种氧化锌纳米颗粒(zinc oxide nanoparticles,ZnO-NPs),以水为溶剂,n(Zn^(2+))∶n(OH^(-))为1∶8、水热温度120℃、恒温6 h条件下,制备的纳米颗粒为球形,采用动态光散射法测得ZnO-NPs的平均粒径为93 nm,多分散系数为0.024。X射线能谱分析、X射线衍射分析、傅里叶变换红外光谱分析等结果显示,所制备的纳米颗粒只含Zn和O两种元素,具有ZnO所特有的晶体结构和化学键特征。抑菌实验结果显示,所制备的ZnO-NPs对细菌和真菌有显著的抑制效果,玉米中添加ZnO-NPs后经过30 d的储藏,与对照组相比其菌落总数明显降低,且减缓了玉米品质劣变。研究结果表明,ZnO-NPs有助于抑制玉米储藏过程中的微生物繁殖,可为实现玉米长期安全储备过程中开发新的微生物抑制剂提供数据支撑。
As one of the main grain reserves in China,maize is subject to mildew due to its high moisture content,rich nutrients and high microbial load,resulting in the production of mycotoxins exceeding the safe limit.In this research,antimicrobial zinc oxide nanoparticles(ZnO-NPs)were prepared by a hydrothermal method under the optimized conditions as follows:using water as the solvent,n(Zn^(2+)):n(OH^(-))of 1:8,water temperature of 120℃,and holding time of 6 h.The nanoparticles were spherical with an average diameter of 93 nm,and polydispersity index(PDI)of 0.024 as determined by dynamic light scattering.Characterization by energy dispersive spectroscopy(EDS),X-ray diffraction(XRD)and Fourier transform infrared spectroscopy(FTIR)showed that the nanoparticles only contained Zn and O elements and had the unique crystal structure and chemical bond characteristics of ZnO.The ZnO-NPs had a significant inhibitory effect on the growth of bacteria and fungi.After 30 days of storage,the total viable count on maize with the ZnO-NPs was significantly lower than that on the control group,and the deterioration of maize quality was slowed down in the ZnO-NPs treatment group.Therefore,the ZnO-NPs could help to inhibit the growth of microorganisms during the storage of maize,which will provide data support for the development of new grain preservatives.
作者
张咚咚
胡思
Zakir HAYAT
宋慧玲
周显青
ZHANG Dongdong;HU Si;Zakir HAYAT;SONG Huiling;ZHOU Xianqing(Grain Storage and Security Engineering Research Center of Education Ministry,College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450001,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2021年第21期137-144,共8页
Food Science
基金
国家自然科学基金青年科学基金项目(32001745)
中国科协第五届青年人才托举工程项目(2019QNRC001)
河南省高等学校重点科研项目(20B55003)。
关键词
水热法
氧化锌纳米颗粒
抗菌活性
玉米储藏
hydrothermal method
ZnO nanoparticles
antibacterial activity
maize storage
